Department of Orthopedic Surgery, College of Medicine, Seoul National University, SMG-SNU Boramae Medical Center, Seoul, Republic of Korea.
Acta Biomater. 2013 Apr;9(4):6177-87. doi: 10.1016/j.actbio.2012.11.030. Epub 2012 Dec 2.
Polyetheretherketone (PEEK) is a material that is widely used in medicine because its mechanical properties show excellent similarity to those of human bone. However, because it is bioinert, PEEK shows limited ability to bind to natural bone tissue. Here, we applied a cold spray method to make a hydroxyapatite (HA)-coated PEEK hybrid material and evaluated its osteointegration in vitro and in vivo. With the cold spray method, the HA coating formed a homogeneous layer and adhered strongly to the PEEK disk implant. When the material was tested in vitro, early cell adhesion and viability improved. Alkaline phosphatase (ALP) activity and calcium concentration were also higher in cells cultured on HA-coated PEEK disks. In addition, the expression of osteoblast differentiation markers, such as ALP, bone sialoprotein and runt-related transcription factor 2, increased in these cells. For the in vivo test, we designed and implanted HA-coated PEEK cylinders into a rabbit ilium model by the press-fit method. The bone-implant contact ratio, trabecular number and trabecular thickness were determined using either three-dimensional microcomputed tomography or general two-dimensional histomorphometric analysis. This report demonstrates that the HA coating on the PEEK implant added with the cold spray method increased biocompatibility in vitro and promoted osteointegration in vivo, which suggests that the HA coating may improve the biofunctionality of various medical devices used in clinical applications.
聚醚醚酮（PEEK）是一种在医学中广泛应用的材料，因为其机械性能与人骨非常相似。然而，由于它是生物惰性的，PEEK 与天然骨组织的结合能力有限。在这里，我们应用冷喷涂方法制备了一种羟基磷灰石（HA）涂层的 PEEK 杂化材料，并评估了其体外和体内的骨整合能力。通过冷喷涂方法，HA 涂层形成了均匀的层并牢固地附着在 PEEK 盘状植入物上。当在体外进行测试时，早期细胞黏附和活力得到了改善。在 HA 涂层的 PEEK 盘上培养的细胞中，碱性磷酸酶（ALP）活性和钙浓度也更高。此外，成骨细胞分化标志物如 ALP、骨涎蛋白和 runt 相关转录因子 2 的表达也在这些细胞中增加。对于体内测试，我们通过压配合法设计并植入了 HA 涂层的 PEEK 圆柱体到兔髂骨模型中。使用三维微计算机断层扫描或常规二维组织形态计量学分析来确定骨-植入物接触比、骨小梁数量和骨小梁厚度。本报告表明，PEEK 植入物上的 HA 涂层通过冷喷涂方法增加了体外的生物相容性，并促进了体内的骨整合，这表明 HA 涂层可能改善各种用于临床应用的医疗设备的生物功能。
