Isaza L, Marulanda M L, López A M
Plant Biotechnology Laboratory, Faculty of Environmental Sciences, Technological University of Pereira, La Julita, Pereira, Colombia.
Genet Mol Res. 2012 Dec 19;11(4):4552-63. doi: 10.4238/2012.November.12.9.
Researchers have classified the Heliconia genus as a group of highly variable and diverse plants. Species and cultivars are visually differentiated primarily on the basis of the color and size of inflorescence bracts. At taxonomic level, flower type (parabolic, sigmoid, or erect) and size are taken into account. The vast morphological diversity of heliconias at intra-specific, intra-population, and varietal levels in central-west Colombia prompted the present study. We characterized the genetic variability of 67 genotypes of cultivated heliconias belonging to Heliconia caribaea Lamarck, H. bihai (L.) L., H. orthotricha L. Andersson, H. stricta Huber, H. wagneriana Petersen, and H. psittacorum L. f., as well as that of several interspecific hybrids such as H. psittacorum L. f. x H. spathocircinata Aristeguieta and H. caribaea Lamarck x H. bihai (L.) L. We also created an approximation to their phylogenetic analysis. Molecular analysis using amplified fragment length polymorphism (AFLP) markers revealed a total of 170 bands. Two large, well-defined groups resulted: the first grouped cultivars of the very closely related H. caribaea and H. bihai species with those of H. orthotricha and H. psittacorum, and the second grouped H. stricta and H. wagneriana cultivars. The lowest percentage of polymorphism was found in H. psittacorum (17.65%) and the highest was in H. stricta (55.88%). Using AFLP, phylogenetic analysis of the species studied revealed the monophyletic origin of the Heliconiaceae family, and identified the Heliconia subgenus as monophyletic while providing evidence of the polyphyletic origin of several representatives of the Stenochlamys subgenus.
研究人员已将蝎尾蕉属归类为一组高度可变且多样的植物。物种和栽培品种主要根据花序苞片的颜色和大小在视觉上加以区分。在分类学层面，会考虑花型（抛物线形、S形或直立形）和大小。哥伦比亚中西部蝎尾蕉在种内、种群内和品种层面存在巨大的形态多样性，这促使了本研究的开展。我们对属于卡里贝蝎尾蕉（Heliconia caribaea Lamarck）、火红蝎尾蕉（H. bihai (L.) L.）、直毛蝎尾蕉（H. orthotricha L. Andersson）、坚挺蝎尾蕉（H. stricta Huber）、瓦氏蝎尾蕉（H. wagneriana Petersen）和鹦喙蝎尾蕉（H. psittacorum L. f.）的67个栽培蝎尾蕉基因型的遗传变异性进行了表征，还对一些种间杂种，如鹦喙蝎尾蕉（H. psittacorum L. f.）× 圆叶蝎尾蕉（H. spathocircinata Aristeguieta）和卡里贝蝎尾蕉（Heliconia caribaea Lamarck）× 火红蝎尾蕉（H. bihai (L.) L.）的遗传变异性进行了表征。我们还对它们的系统发育分析进行了近似研究。使用扩增片段长度多态性（AFLP）标记进行的分子分析共揭示了170条带。结果形成了两个大型且界限分明的组：第一组将亲缘关系非常密切的卡里贝蝎尾蕉和火红蝎尾蕉品种与直毛蝎尾蕉和鹦喙蝎尾蕉的品种归为一类，第二组将坚挺蝎尾蕉和瓦氏蝎尾蕉品种归为一类。在鹦喙蝎尾蕉中发现的多态性百分比最低（17.65%），在坚挺蝎尾蕉中最高（55.88%）。利用AFLP对所研究的物种进行系统发育分析，揭示了蝎尾蕉科的单系起源，并确定蝎尾蕉亚属为单系，同时为窄瓣蝎尾蕉亚属几个代表种的多系起源提供了证据。
