3-羟基-3-甲基戊二酰辅酶A还原酶基因的两个新单核苷酸多态性与鸡的生长和肉质性状相关。

Two novel SNPs of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene associated with growth and meat quality traits in the chicken.

作者信息

Wei Y, Zhu S K, Zhang S, Han R L, Tian Y D, Sun G R, Kang X T

机构信息

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

出版信息

Genet Mol Res. 2012 Sep 18;11(4):4765-74. doi: 10.4238/2012.November.12.10.

DOI:10.4238/2012.November.12.10

PMID:23212401

Abstract

The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is rate-limiting for metabolism of cholesterol; it plays an important role in endogenous cholesterol biosynthesis. We used DNA sequencing technology and created restriction site PCR-RFLP to detect HMGCR SNPs in an F(2) resource population of Gushi chicken and Anka broilers. We found a G/T mutation (Gln/His) in exon 17 and a T/C mutation (Pro/Pro) in exon 18. Based on association analysis of these HMGCR polymorphisms in 864 Gushi/Anka F(2) hybrids, these two mutations have significant effects on growth, carcass, meat quality, and lipid concentration.

摘要

3-羟基-3-甲基戊二酰辅酶A还原酶（HMGCR）是胆固醇代谢的限速酶；它在内源性胆固醇生物合成中起重要作用。我们使用DNA测序技术并创建限制性位点PCR-RFLP来检测固始鸡和安卡肉鸡F(2)资源群体中的HMGCR单核苷酸多态性（SNP）。我们在第17外显子中发现了一个G/T突变（Gln/His），在第18外显子中发现了一个T/C突变（Pro/Pro）。基于对864只固始/安卡F(2)杂交鸡中这些HMGCR多态性的关联分析，这两个突变对生长、胴体、肉质和脂质浓度有显著影响。