College of Livestock Husbandry and Veterinary Engineering, Henan Research Center of Breeding Resources for Poultry, Henan Agricultural University, No 95 Wenhua Road, Zhengzhou, 450002 Henan, People's Republic of China.
Mol Biol Rep. 2012 Mar;39(3):3153-60. doi: 10.1007/s11033-011-1081-y. Epub 2011 Jul 15.
The PR domain containing 16 (PRDM16) is a member of the Prdm family, and is known to regulate cell differentiation. In the present study, DNA pool sequencing methods were employed to screen genetic variations in the chicken PRDM16 gene. The results revealed four novel single nucleotide polymorphisms (SNPs): NC_006108.2: g.92188G>A, XM_417551: c.1161C>T (Ala/Ala, 387aa), c.1233C>T (Ser/Ser, 411aa) and c.1433G>A (Ser/Asn, 478aa). The BglI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect c.1161C>T, while HhaI Forced PCR-RFLP methods were used to detect 1233C>T and c.1433G>A in 964 chickens. The chickens comprised 38 grandparents, 66 F(1) parents and 860 F(2) birds derived from an F(2) resource population of Gushi chickens crossed with Anka broilers. The associations of the polymorphisms in the chicken PRDM16 gene with performance traits were analyzed in the 860 F(2) chickens. The results indicated that the three SNPs were significantly associated with growth, fatness and meat quality traits in the chickens. In particular, the polymorphisms of the missense SNP (c.1433G>A) had positive effects on chicken body weight and body size at different stages. It affected also fatness traits significantly. Comparison of the different genotypes of c.1433G>A showed that the GG genotype favored chicken growth and fatness traits.
PR 结构域包含蛋白 16（PRDM16）是 Prdm 家族的成员，已知其可调节细胞分化。本研究采用 DNA 池测序方法筛选鸡 PRDM16 基因的遗传变异。结果显示，发现了 4 个新的单核苷酸多态性（SNP）：NC_006108.2：g.92188G>A、XM_417551：c.1161C>T（Ala/Ala，387aa）、c.1233C>T（Ser/Ser，411aa）和 c.1433G>A（Ser/Asn，478aa）。BglI 聚合酶链反应-限制性片段长度多态性（PCR-RFLP）用于检测 c.1161C>T，而 HhaI 强制 PCR-RFLP 方法用于检测 1233C>T 和 c.1433G>A 在 964 只鸡中。这些鸡包括 38 个祖代、66 个 F1 父母和 860 个来自固始鸡与安卡肉鸡杂交的 F2 资源群体的 F2 代鸟。分析了鸡 PRDM16 基因多态性与性能性状的相关性。结果表明，这 3 个 SNP 与鸡的生长、肥胖和肉质性状显著相关。特别是错义 SNP（c.1433G>A）的多态性对鸡不同阶段的体重和体型有积极影响。它还显著影响肥胖性状。不同基因型 c.1433G>A 的比较表明，GG 基因型有利于鸡的生长和肥胖性状。
