College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea.
Arch Pharm Res. 2012 Nov;35(11):2007-12. doi: 10.1007/s12272-012-1118-3. Epub 2012 Dec 4.
Although some anti-allergic activities of the rhizome of Atractylodes japonica have been previously reported, the active principle(s) for anti-allergic action is not fully elucidated and the effect of this plant material on atopic dermatitis (AD) is not known. In this study, the 70% ethanol extract of the rhizome of A. japonica was found to significantly inhibit 5-lipoxygenase (5-LOX)-catalyzed leukotrienes (LT) production from rat basophilic leukemia (RBL)-1 cells. From the extract of A. japonica, three major sesquiterpene derivatives including atractylenolide I, atractylenolide III and eudesma-4,7-dien-8-one were successfully isolated. Among these compounds, only atractylenolide I was shown to strongly inhibit 5-LOX from RBL-1 cells (IC(50) = 18.6 μM). To evaluate the effects of experimental AD, the ethanol extract of A. japonica (200 mg/day) was administered orally to hapten-treated NC/Nga mice which is an animal model of AD. It was firstly found that the extract significantly inhibited AD-like symptoms in mice, as judged by severity score and scratching behavior. Taken together, it is concluded that A. japonica possesses the inhibitory activity on 5-LOX and an animal model of AD, and atractylenolide I may contribute, at least in part, to these anti-allergic actions of A. japonica.
虽然之前已经报道了白术根茎的一些抗过敏活性，但抗过敏作用的活性成分尚未完全阐明，并且这种植物材料对特应性皮炎（AD）的影响尚不清楚。在这项研究中，发现白术根茎的 70%乙醇提取物可显著抑制大鼠嗜碱性白血病（RBL）-1 细胞中 5-脂氧合酶（5-LOX）催化的白三烯（LT）的产生。从白术的提取物中，成功分离出三种主要的倍半萜衍生物，包括白术内酯 I、白术内酯 III 和桉叶-4,7-二烯-8-酮。在这些化合物中，只有白术内酯 I 显示出强烈抑制 RBL-1 细胞 5-LOX 的作用（IC50 = 18.6 μM）。为了评估实验性 AD 的效果，白术乙醇提取物（200mg/天）口服给予变应原处理的 NC/Nga 小鼠，这是 AD 的动物模型。研究人员首先发现，提取物可显著抑制小鼠的 AD 样症状，其严重程度评分和搔抓行为均有所下降。综上所述，白术具有抑制 5-LOX 的活性，并且对 AD 动物模型有抑制作用，白术内酯 I 可能至少部分参与了白术的这些抗过敏作用。
