Xu Meihong, Ma Yi, Xu Linlin, Xu Yajun, Li Yong
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, People's Republic of China.
Birth Defects Res B Dev Reprod Toxicol. 2012 Dec;95(6):460-6. doi: 10.1002/bdrb.21033. Epub 2012 Dec 4.
With the multifaceted activities of nucleotides, there is a history of safe consumption of dietary nucleotides (NTs) in the human diet. This study investigated the multigenerations cumulative toxicity on rats' development after weaning. Weaning rats (F0) were fed with NTs at the dosage of 0.01, 0.04, 0.16, 0.64, and 1.28% (wt/wt) for 90 days and then mated for 1:1 pattern. The offspring was F1. F1 rats were fed with NTs for 90 days after weaning. Afterwards, F1 go on to the second reproductive part. We repeated the above process, until F3 rats were born. We observed the weight, food consumption in the whole experiment, and detected the blood indicators and organ pathology at the terminal. No abnormal reaction, behavior disorder, and organ pathology related to toxic symptom were observed in NTs-treated groups. Weight gain and diet utilization ratio of males in each NTs group had significant increase after weaning (p < 0.05). With the exception of decrease in uric acid (p < 0.05) of NTs male, there were no differences between the control and NTs groups in blood indicators. NTs could promote early growth and development of rats after weaning, especially in males.
由于核苷酸具有多方面的活性，人类饮食中对膳食核苷酸（NTs）的安全食用已有一定历史。本研究调查了断奶后NTs对大鼠发育的多代累积毒性。将断奶大鼠（F0）分别以0.01%、0.04%、0.16%、0.64%和1.28%（重量/重量）的剂量喂食NTs 90天，然后按1:1的模式进行交配。子代即为F1。F1大鼠断奶后喂食NTs 90天。之后，F1进入第二次繁殖阶段。我们重复上述过程，直至F3大鼠出生。在整个实验过程中，我们观察了大鼠的体重、食物消耗量，并在实验末期检测了血液指标和器官病理情况。在接受NTs处理的组中，未观察到与毒性症状相关的异常反应、行为紊乱和器官病理变化。各NTs组雄性大鼠断奶后的体重增加和饮食利用率均显著提高（p<0.05）。除了NTs组雄性大鼠尿酸降低（p<0.05）外，对照组和NTs组在血液指标上无差异。NTs可促进大鼠断奶后的早期生长发育，尤其是对雄性大鼠。
