Yu Jing, Qi Yue, Luo Gang, Duan Hong-quan, Zhou Jing
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
Zhong Yao Cai. 2012 May;35(5):796-9.
To optimize the extraction method of essential oil in Pogostemon cablin and analyze its inhibitory activity against Hela cell proliferation.
The Pogostemon cablin was treated by hemicellulase before steam distillation. The enzyme dosage, treatment time, treatment temperature, pH were optimized through orthogonal experimental design. The components of essential oil were identified by gas chromatography-mass spectrometry (GC-MS). Inhibitory activity of patchouli oil against Hela cell proliferation was determined by MTP method.
The optimum extraction process was as follows: pH 4.5, temperature 45 degrees C, the ratio of hemicellulase to Pogostemon cablin was 1% and enzymatic hydrolysis for 1.0 hour. Extraction ratio of the patchouli oil in steam distillation and hemicellulase extraction method was 2.2220 mg/g, 3.1360 mg/g respectively. Patchouli oil could inhibit Hela cell proliferation. IC50 of the patchouli oil in steam distillation and hemicellulase extraction method was 12.2 +/- 0.46 microg/mL and 0.36 +/- 0.03 microg/mL respectively.
In comparison with steam distillation method, extraction ratios of essential oil and the inhibitory activity against Hela cell proliferation can be increased by the hemicellulase extraction method.
优化广藿香挥发油的提取方法，并分析其对Hela细胞增殖的抑制活性。
采用半纤维素酶对广藿香进行预处理后再进行水蒸气蒸馏。通过正交试验设计优化酶用量、处理时间、处理温度、pH值。采用气相色谱-质谱联用（GC-MS）法鉴定挥发油的成分。采用MTT法测定广藿香油对Hela细胞增殖的抑制活性。
最佳提取工艺为：pH值4.5，温度45℃，半纤维素酶与广藿香的比例为1%，酶解1.0小时。水蒸气蒸馏法和半纤维素酶提取法的广藿香油提取率分别为2.2220mg/g、3.1360mg/g。广藿香油能抑制Hela细胞增殖。水蒸气蒸馏法和半纤维素酶提取法的广藿香油IC50分别为12.2±0.46μg/mL和0.36±0.03μg/mL。
与水蒸气蒸馏法相比，半纤维素酶提取法可提高挥发油的提取率及对Hela细胞增殖的抑制活性。
