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[曝气生物滤池脱氮性能及反硝化细菌群落结构特征研究]

[Study on the nitrogen removal performance and the characteristics of denitrification bacterial community structure of biological aerated filter].

作者信息

Peng Xiao-lan, Liu Cong, Chen Lü-jun

机构信息

School of Environment, Tsinghua University, Beijing 100084, China.

出版信息

Huan Jing Ke Xue. 2012 Aug;33(8):2701-8.

PMID:23213893
Abstract

The nitrogen removal performance and the denitrification bacterial community structure of two pre-denitrification biological aeration filter (BAF) processes with different media combinations, the ceramic-zeolite BAF process (C-Z BAF) and the zeolite-ceramic BAF process (Z-C BAF), were compared at different C/N ratios. The results showed that the average TN removal efficiency of Z-C BAF was higher than that of C-Z BAF, and the advantage was more notable at low C/N ratio. When the C/N ratio decreased to 3.3, the average TN removal efficiency of Z-C BAF was 20% higher than that of C-Z BAF. Comparing the microbial community structure of the two combinations at different C/N ratios, it was found that the richness and diversity of denitrification bacterial community decreased significantly with the decrease of C/N ratio. At low C/N ratio, within the whole oxic filter of Z-C BAF, nosZ genetic T-RFs were detected with more than 20% relative abundance, whereas no nosZ genetic T-RFs were detected at the upper oxic filter of C-Z BAF by PCR amplification. The results indicated that the simultaneous nitrification and denitrification function of Z-C BAF was better than that of C-Z BAF. At low C/N ratio, the nitrogen removal efficiency of Z-C BAF was obviously higher than that of C-Z BAF. Thus, compared to C-Z BAF, Z-C BAF had greater potential on the treatment of wastewater with low organic concentration and low C/N ratio.

摘要

比较了两种不同滤料组合的前置反硝化曝气生物滤池(BAF)工艺,即陶瓷-沸石曝气生物滤池工艺(C-Z BAF)和沸石-陶瓷曝气生物滤池工艺(Z-C BAF)在不同碳氮比下的脱氮性能及反硝化细菌群落结构。结果表明,Z-C BAF的总氮平均去除效率高于C-Z BAF,且在低碳氮比时优势更显著。当碳氮比降至3.3时,Z-C BAF的总氮平均去除效率比C-Z BAF高20%。比较两种组合在不同碳氮比下的微生物群落结构发现,反硝化细菌群落的丰富度和多样性随碳氮比的降低而显著下降。在低碳氮比时,Z-C BAF整个好氧滤池中检测到相对丰度超过20%的nosZ基因T-RFs,而通过PCR扩增在C-Z BAF的上部好氧滤池中未检测到nosZ基因T-RFs。结果表明,Z-C BAF的同步硝化反硝化功能优于C-Z BAF。在低碳氮比时,Z-C BAF的脱氮效率明显高于C-Z BAF。因此,与C-Z BAF相比,Z-C BAF在处理低有机浓度和低碳氮比废水方面具有更大潜力。

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