Zhang Qian, Ji Fang-Ying, Fu Xu-Fang, Chen Qing-Kong
School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China.
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Huan Jing Ke Xue. 2018 Jul 8;39(7):3297-3305. doi: 10.13227/j.hjkx.201712075.
To solve the problems of high-energy consumption, complex processes, and low nitrogen removal efficiency in the currently available low carbon source wastewater treatment processes, a novel coagulation sedimentation/post solid-phase denitrification biofilter process (CS-BAF-SPDB) was proposed. The effect of gas/water ratio on nitrogen removal efficiency of the CS-BAF-SPDB was studied. The changes in the microbial community structure along the varying gas/water ratio were investigated, and the functional bacteria were identified using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that, to realize favorable nitrifying and denitrifying performance simultaneously in the BAF-SPDB unit, the gas/water ratio of the BAF should be set at 4∶1. In addition, the influence of the macro operational parameters on the performance of the BAF and SPDB had a direct relationship with the dynamic changes in the micro microbial community. The influence of gas/water ratio on nitrification performance in the BAF was mainly embodied in the changes of composition, amount, and activity of ammonia-oxidizing bacteria and and nitrite-oxidizing bacteria sp. Nm47, whereas that on denitrification performance in the SPDB was mainly embodied in the changes of composition and amount of solid carbon substrate degrading denitrifying bacteria sp. and AT3-03 and heterotrophic denitrifying bacteria , , and .
为解决现有低碳源污水处理工艺中存在的高能耗、流程复杂和脱氮效率低等问题，提出了一种新型的混凝沉淀/后置固相反硝化生物滤池工艺（CS-BAF-SPDB）。研究了气水比对CS-BAF-SPDB脱氮效率的影响。考察了沿不同气水比变化的微生物群落结构变化，并采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）鉴定了功能细菌。结果表明，为使BAF-SPDB单元同时实现良好的硝化和反硝化性能，BAF的气水比应设定为4∶1。此外，宏观运行参数对BAF和SPDB性能的影响与微生物群落的动态变化有直接关系。气水比对BAF中硝化性能的影响主要体现在氨氧化细菌和亚硝酸盐氧化细菌Nm47的组成、数量和活性变化上，而对SPDB中反硝化性能的影响主要体现在固体碳源降解反硝化细菌和AT3-03以及异养反硝化细菌、和的组成和数量变化上。
