Qi Xu-liang, Liu Xiang, Liu Bo, Wang Lin, Wang Xiao-chun, Fang Chao
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.
Huan Jing Ke Xue. 2012 Aug;33(8):2747-51.
Crude laccase from Trametes versicolor was immobilized onto anion exchange resin D201 by three methods, i.e., direct electrostatic adsorption (D201-Lac-I), crosslinking after electrostatic adsorption (D201-Lac-II) and electrostatic adsorption after treating D201 with glutaraldehyde (D201-Lac-III). Compared to direct electrostatic adsorption, the immobilized laccase amount of D201-Lac-II increased by 4.65 times but the laccase activity was decreased to 4.8%, while the laccase activity on D201-Lac-III increased by 2.99 times, with the immobilization amount decreased to 51%. Shadows of laccase aggregation on D201-Lac-III were found by transmission electron microscopy (TEM). Continuous batch decoloration of malachite green demonstrated that the decoloration efficiency of D201-Lac-III remained in the range of 40% to 55% for more than 210 hours, in addition, the enzyme activity on D201-Lac-III maintained unchanged while the activity of free laccase declined to less than 20% under the same condition. All of the results above indicated that D201-Lac-III had a significantly enhanced stability and good reusability. Considering the low price and simple production procedure of crude laccase, D201-Lac-III could be promising for water treatment purpose.
采用三种方法将云芝粗漆酶固定在阴离子交换树脂D201上，即直接静电吸附法（D201-Lac-I）、静电吸附后交联法（D201-Lac-II）和用戊二醛处理D201后的静电吸附法（D201-Lac-III）。与直接静电吸附相比，D201-Lac-II的固定化漆酶量增加了4.65倍，但漆酶活性降至4.8%，而D201-Lac-III上的漆酶活性增加了2.99倍，固定化量降至51%。通过透射电子显微镜（TEM）发现了D201-Lac-III上漆酶聚集的阴影。孔雀石绿的连续分批脱色表明，D201-Lac-III的脱色效率在210多个小时内保持在40%至55%的范围内，此外，在相同条件下，D201-Lac-III上的酶活性保持不变，而游离漆酶的活性降至20%以下。上述所有结果表明，D201-Lac-III具有显著增强的稳定性和良好的可重复使用性。考虑到粗漆酶价格低廉且生产工艺简单，D201-Lac-III在水处理方面具有广阔的应用前景。
