多孔硅中的稳定漆酶在流化床反应器中高效生物转化双酚 A。
High efficiency biotransformation of bisphenol A in a fluidized bed reactor using stabilized laccase in porous silica.
机构信息
Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China; College of Environmental Science and Engineering, Jilin Normal University, 1301 Haifeng Road, Siping 136000, China.
Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China.
出版信息
Enzyme Microb Technol. 2019 Jul;126:1-8. doi: 10.1016/j.enzmictec.2019.03.006. Epub 2019 Mar 28.
We developed a high efficiency bisphenol A (BPA) treating system in a fluidized bed reactor (FBR) using stabilized laccase (Lac) in mesoporous silica (SER/Lac). The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking. When the SER/Lac was used for BPA biotransformation in a batch reaction, higher efficiency was achieved than the free or adsorbed Lac (ADS/Lac). Also, the SER/Lac presented better reusability compared to ADS/Lac, and it could be reused for three times without decrease of biotransformation efficiency, and half of it was remained after ten times use. Due to great stability and robustness property, SER/Lac was successfully applied for high efficiency and continuous BPA treatment in FBR with better performance than the batch reaction.
我们在流化床反应器（FBR）中使用固定化漆酶（Lac）在介孔硅（SER/Lac）中开发了一种高效双酚 A（BPA）处理系统。通过戊二醛交联多孔硅中的各酶制备的 SER/Lac，在各种 pH 值和温度下具有比游离 Lac 更高的稳定性，在摇床中也具有更好的稳定性。当 SER/Lac 用于分批反应中的 BPA 生物转化时，与游离或吸附的 Lac（ADS/Lac）相比，效率更高。此外，与 ADS/Lac 相比，SER/Lac 的可重复使用性更好，它可以重复使用三次而不会降低生物转化效率，并且在使用十次后仍保留一半。由于具有良好的稳定性和鲁棒性，SER/Lac 成功地应用于 FBR 中进行高效和连续的 BPA 处理，其性能优于分批反应。
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