Ye Yun-di, Sun Shui-yu, Zheng Li, Liu Bao-jian, Xu Yan-bin, Zhan Xing-xing, Liu Jing-yong
College of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China.
Huan Jing Ke Xue. 2012 Aug;33(8):2780-5.
In order to evaluate the function of sludge aerobic/anoxic digestibility by ultrasonic pretreatment. The SS, VSS and hydrolytic enzyme activities (amylase, glucosidase, protease, phosphatase) were measured before and after ultrasonic pretreatment (28 kHz, 0.15 kW x L(-1), 10 min). The results showed that the performances of aerobic/anoxic were greatly improved after ultrasonic pretreatment, the removal efficiency of VSS went to 44.3%, 7.8% better than of traditional aerobic/anoxic digestion. The variational trend of sludge hydrolytic enzyme activities increased firstly and then fell off during 13d digestion, the maximum of amylase activity and glucosidase activity in ultrasonic sludge, appeared in the 5 d, amylase activity was 0.104 micromol x g(-1) and glucosidase activity was 0.637 (micromol x g(-1). The maximum of intracellular protease activity and extracellular proteases activity in ultrasonic sludge, appeared in the 7 d, intracellular protease activity was 23.68 micromol x g(-1), higher than extracellular proteases activity, and it was playing a leading role in sludge digestion. The acid phosphatase activity of ultrasonic sludge was higher than the control sludge, and the alkaline phosphatase was sensitive to environment. So the alkaline phosphatase activity reduced when the internal properties of sludge was changed.
为了评估超声预处理对污泥好氧/缺氧消化性能的影响。在超声预处理（28 kHz，0.15 kW·L⁻¹，10 min）前后测定了污泥的悬浮固体（SS）、挥发性悬浮固体（VSS）和水解酶活性（淀粉酶、葡萄糖苷酶、蛋白酶、磷酸酶）。结果表明，超声预处理后污泥的好氧/缺氧消化性能得到显著改善，VSS去除率达到44.3%，比传统好氧/缺氧消化提高了7.8%。在13天的消化过程中，污泥水解酶活性呈先升高后下降的变化趋势，超声处理后的污泥中淀粉酶活性和葡萄糖苷酶活性在第5天达到最大值，淀粉酶活性为0.104 μmol·g⁻¹，葡萄糖苷酶活性为0.637 μmol·g⁻¹。超声处理后的污泥中细胞内蛋白酶活性和细胞外蛋白酶活性在第7天达到最大值，细胞内蛋白酶活性为23.68 μmol·g⁻¹，高于细胞外蛋白酶活性，在污泥消化中起主导作用。超声处理后的污泥酸性磷酸酶活性高于对照污泥，碱性磷酸酶对环境敏感。因此，当污泥内部性质发生变化时，碱性磷酸酶活性降低。
