School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; School of Environment & Resource, Renmin University of China, Beijing 100872, China.
Bioresour Technol. 2013 Oct;146:758-761. doi: 10.1016/j.biortech.2013.07.087. Epub 2013 Jul 26.
In this study, the effects of endogenous amylase, endogenous protease and combined amylase/protease pretreatment of sludge were studied to enhance the efficiency of sludge anaerobic digestion. These enzymes were obtained from bacterial fermentation and bacteria were separated from the sludge. All treatments improved sludge solubilization and acidification but had little influence on the floc sizes. In terms of sludge solubilization and acidification amylase was better than protease or mixed enzyme. After 7 h endogenous amylase treatment, the supernatant soluble chemical oxygen demand and volatile fatty acids concentration increased by 78.2% and 129.6%, respectively. But, in terms of anaerobic biodegradability, the best result was obtained with combined enzyme treatment, biogas production increased by 23.1% compared to the control after 11 days of anaerobic digestion. Scanning electron micrographs observation and particle size analysis revealed that the most important mechanism for the enzyme treatment of sludge might be solubilization of extracellular polymeric substances.
在这项研究中，研究了内源性淀粉酶、内源性蛋白酶和污泥的联合淀粉酶/蛋白酶预处理对提高污泥厌氧消化效率的影响。这些酶是从细菌发酵中获得的，并从污泥中分离出来。所有处理都提高了污泥的溶解和酸化效率，但对絮体大小几乎没有影响。在污泥溶解和酸化方面，淀粉酶优于蛋白酶或混合酶。经过 7 小时的内源性淀粉酶处理后，上清液中可溶解化学需氧量和挥发性脂肪酸浓度分别增加了 78.2%和 129.6%。但是，就厌氧生物降解性而言，经过 11 天的厌氧消化，联合酶处理的效果最好，沼气产量比对照增加了 23.1%。扫描电子显微镜观察和颗粒大小分析表明，酶处理污泥的最重要机制可能是细胞外聚合物的溶解。
