Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University, Seoul, Korea.
J Pharm Pharmacol. 2013 Jan;65(1):142-8. doi: 10.1111/j.2042-7158.2012.01570.x. Epub 2012 Aug 15.
The root of Astragalus membranaceus, regarded as a tonic in traditional Korean medicine, has been prescribed for long periods to treat chronic illness by boosting the immune system. Ultraviolet (UV) irradiation causes damage to skin connective tissue by degrading collagen, which is a major structural component of the extracellular matrix. Such damage is considered to be a cause of the wrinkling observed in premature ageing of the skin. This study has investigated the photo-protective effect of A. membranaceus on UVB radiation-induced activation of nuclear factor kappa-B (NF-κB) activity in human dermal fibroblasts.
HS68 fibroblast cells cultured with various concentrations of A. membranaceus were exposed to UVB (40 mJ/cm²). Activation of NF-κB P65 and expression of matrix metalloproteinase-1 (MMP-1) and type 1 procollagen were measured by Western blotting. Translocation of NF-κB P65 and MMP-1 regulation were also examined by immunocytochemistry.
Western blotting and immunocytochemistry results showed that A. membranaceus inhibited UVB-induced translocation of NF-κB P65 and MMP-1 expression. The data suggested that A. membranaceus restored type 1 procollagen synthesis by inhibiting NF-κB P65 activity and MMP-1 expression in UVB-exposed human dermal fibroblasts.
A. membranaceus is a candidate for use in skin protection from UVB-induced skin inflammation and photoageing.
作为传统韩国医学中的补品，黄芪的根部已被长期用于通过增强免疫系统来治疗慢性疾病。紫外线 (UV) 照射会通过降解胶原蛋白而对皮肤结缔组织造成损伤，而胶原蛋白是细胞外基质的主要结构成分。这种损伤被认为是皮肤过早衰老时出现皱纹的原因之一。本研究调查了黄芪对 UVB 辐射诱导的人真皮成纤维细胞核因子 kappa-B (NF-κB) 活性的光保护作用。
用不同浓度的黄芪培养 HS68 成纤维细胞，然后用 UVB（40 mJ/cm²）照射。通过 Western blot 测定 NF-κB P65 的激活以及基质金属蛋白酶-1 (MMP-1) 和 I 型前胶原的表达。还通过免疫细胞化学检查 NF-κB P65 的易位和 MMP-1 的调节。
Western blot 和免疫细胞化学结果表明，黄芪抑制了 UVB 诱导的 NF-κB P65 易位和 MMP-1 表达。数据表明，黄芪通过抑制 NF-κB P65 活性和 MMP-1 在 UVB 暴露的人真皮成纤维细胞中的表达，恢复了 I 型前胶原的合成。
黄芪是一种可用于保护皮肤免受 UVB 诱导的皮肤炎症和光老化的候选药物。
