Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
Food Funct. 2014 Feb;5(2):265-74. doi: 10.1039/c3fo60418g.
Panax ginseng Meyer (Ginseng) has been used widely in traditional herbal medicine because of its pharmacological activities. In this study, we tested the ability of an enzyme-modified ginseng extract (EG) to protect the skin against ultraviolet B (UVB)-induced damage using cultured human dermal fibroblasts and hairless mice. EG, an extract which is rich in the active compound ginsenoside F2, and purified ginsenoside F2 were used in these experiments. The ginsenoside content of EG was measured by liquid chromatography-mass spectrometry (LC-MS). The potential of EG to reduce UVB-induced skin damage was investigated by determining the levels of procollagen type I and metalloproteinase-1 (MMP-1) after UVB irradiation in human dermal fibroblasts and by examining the levels of hydration, thickness, and density of collagen fibers in the UVB-exposed dorsal skin of hairless mice. LC-MS analysis detected a difference in the ginsenoside content between normal white ginseng and enzyme-modified ginseng. In UVB-irradiated human dermal fibroblasts treated with EG, MMP-1 production considerably decreased without cell toxicity. Furthermore, topical application of EG showed significant reductions in skin dryness, thickness, and fragmented collagen fibers in UVB-exposed hairless mice. Ginsenoside F2, an active component of EG, increased procollagen type I production and decreased MMP-1 secretion in UV-irradiated human dermal fibroblasts. EG and ginsenoside F2 are potentially useful for the prevention and treatment of UVB-induced skin damage.
人参（Panax ginseng Meyer）由于其药理学活性，已被广泛应用于传统草药医学。在这项研究中，我们使用培养的人真皮成纤维细胞和无毛小鼠来测试酶修饰人参提取物（EG）保护皮肤免受紫外线 B（UVB）损伤的能力。EG 是一种富含活性化合物人参皂苷 F2 的提取物，以及纯化的人参皂苷 F2 用于这些实验。通过液相色谱-质谱（LC-MS）测量 EG 中的人参皂苷含量。通过测定 UVB 照射后人真皮成纤维细胞中 I 型前胶原和金属蛋白酶-1（MMP-1）的水平，以及检查 UVB 暴露的无毛小鼠背部皮肤中胶原纤维的水合、厚度和密度，研究 EG 减少 UVB 诱导的皮肤损伤的潜力。LC-MS 分析检测到正常白参和酶修饰参之间的人参皂苷含量存在差异。在 EG 处理的经 UVB 照射的人真皮成纤维细胞中，MMP-1 的产生明显减少而没有细胞毒性。此外，EG 的局部应用在 UVB 暴露的无毛小鼠中显示出皮肤干燥、厚度和碎片化胶原纤维的显著减少。EG 中的活性成分人参皂苷 F2 增加了经 UV 照射的人真皮成纤维细胞中 I 型前胶原的产生，并减少了 MMP-1 的分泌。EG 和人参皂苷 F2 可能有助于预防和治疗 UVB 诱导的皮肤损伤。
