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禽肿瘤病毒gag和pol基因产物的胰蛋白酶肽分析。

Tryptic peptide analysis of avian oncovirus gag and pol gene products.

作者信息

Rettenmier C W, Karess R E, Anderson S M, Hanafusa H

出版信息

J Virol. 1979 Oct;32(1):102-13. doi: 10.1128/JVI.32.1.102-113.1979.

DOI:10.1128/JVI.32.1.102-113.1979
PMID:232163
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC353532/
Abstract

Radiolabeled tryptic peptides of the gag and pol gene products of avian oncoviruses were examined. This analysis included Rous-associated virus 2 structural proteins and the Pr76gag and P180gag-pol proteins in Rous-associated virus 2-infected chicken embryo cells. The methionine- and cysteine-containing tryptic peptides of virion internal structural proteins were present in both Pr76gag and P180gag-pol, suggesting that there was no loss of gag gene-coding sequences during the generation of P180gag-pol. No overlap of gag and pol gene structural information was detected. Analysis of intermediates in the processing of Pr76gag and translation inhibition mapping with pactamycin yielded the following order of structural proteins within the Rous-associated virus 2 Pr76gag precursor: NH2-p19-p12-p27-p15-COOH. The gag and pol sequences missing in the endogenous gsmp120 protein of uninfected gs+ chicken cells were identified by comparison with those of Rous-associated virus 2 P180gag-pol.

摘要

对禽肿瘤病毒gag和pol基因产物的放射性标记胰蛋白酶肽进行了检测。该分析包括劳斯相关病毒2的结构蛋白以及劳斯相关病毒2感染的鸡胚细胞中的Pr76gag和P180gag-pol蛋白。病毒粒子内部结构蛋白含甲硫氨酸和半胱氨酸的胰蛋白酶肽在Pr76gag和P180gag-pol中均有存在，这表明在P180gag-pol产生过程中gag基因编码序列没有丢失。未检测到gag和pol基因结构信息的重叠。对Pr76gag加工中间体的分析以及用放线菌酮进行的翻译抑制图谱分析得出了劳斯相关病毒2 Pr76gag前体中结构蛋白的以下顺序：NH2-p19-p12-p27-p15-COOH。通过与劳斯相关病毒2 P180gag-pol的序列进行比较，确定了未感染的gs+鸡细胞内源性gsmp120蛋白中缺失的gag和pol序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/01ff51fa3735/jvirol00190-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/27721a720a2c/jvirol00190-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/4c60da811f27/jvirol00190-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/8e0e04421c9e/jvirol00190-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/73246d81663f/jvirol00190-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/999564443dfb/jvirol00190-0116-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/e04315bd377b/jvirol00190-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/110fde1caf3d/jvirol00190-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/f0145184facb/jvirol00190-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/01ff51fa3735/jvirol00190-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/27721a720a2c/jvirol00190-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/4c60da811f27/jvirol00190-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/8e0e04421c9e/jvirol00190-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/73246d81663f/jvirol00190-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/999564443dfb/jvirol00190-0116-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/e04315bd377b/jvirol00190-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/110fde1caf3d/jvirol00190-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/f0145184facb/jvirol00190-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edd5/353532/01ff51fa3735/jvirol00190-0122-a.jpg

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