Jove M A, Vassalli L, Raslan W, Applebaum E L
Department of Otolaryngology--Head and Neck Surgery, University of Illinois College of Medicine, Chicago 60612.
Am J Otolaryngol. 1990 Jan-Feb;11(1):5-9. doi: 10.1016/0196-0709(90)90163-p.
Previous studies have shown that propylene glycol causes inflammatory changes and cholesteatoma when applied to chinchilla middle ears. Vitamin A and synthetic analogues are essential for the normal differentiation of epithelial tissues. The purpose of this study was to determine whether the administration of isotretinoin to chinchillas would prevent propylene glycol exposure from inducing middle ear cholesteatomas. Sixteen chinchillas received 90% propylene glycol to the left middle ear and normal saline to the right. Half the animals were placed in the experimental group and received a daily dose of isotretinoin of 2 mg/kg for 7 days prior to propylene glycol administration and then for 6 weeks until killed. At 6 weeks, cholesteatoma was found in six of eight ears treated with propylene glycol in animals receiving isotretinoin. Two animals in the control group died. Three of the remaining eight had cholesteatoma. No ears treated with saline had cholesteatoma. We conclude that isotretinoin, in our chinchilla model, does not prevent propylene glycol-induced cholesteatoma formation.
先前的研究表明，将丙二醇应用于豚鼠中耳时会引起炎症变化和胆脂瘤。维生素A及其合成类似物对于上皮组织的正常分化至关重要。本研究的目的是确定给豚鼠施用异维A酸是否能预防丙二醇暴露诱导中耳胆脂瘤的形成。16只豚鼠左耳中耳接受90%的丙二醇，右耳接受生理盐水。一半动物被置于实验组，在给予丙二醇前7天每天接受2mg/kg的异维A酸剂量，然后持续6周直至处死。6周时，接受异维A酸的动物中，用丙二醇处理的8只耳朵中有6只发现了胆脂瘤。对照组中有2只动物死亡。其余8只中的3只患有胆脂瘤。用生理盐水处理的耳朵未出现胆脂瘤。我们得出结论，在我们的豚鼠模型中，异维A酸不能预防丙二醇诱导的胆脂瘤形成。
