Division of Parasitology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
Malar J. 2012 Dec 6;11:407. doi: 10.1186/1475-2875-11-407.
Serial blood passage of Plasmodium increases virulence, whilst mosquito transmission inherently regulates parasite virulence within the mammalian host. It is, therefore, imperative that all aspects of experimental malaria research are studied in the context of the complete Plasmodium life cycle.
Plasmodium chabaudi chabaudi displays many characteristics associated with human Plasmodium infection of natural mosquito vectors and the mammalian host, and thus provides a unique opportunity to study the pathogenesis of malaria in a single infection setting. An optimized protocol that permits efficient and reproducible vector transmission of P. c. chabaudi via Anopheles stephensi was developed.
This protocol was utilized for mosquito transmission of genetically distinct P. c. chabaudi isolates, highlighting differential parasite virulence within the mosquito vector and the spectrum of host susceptibility to infection initiated via the natural route, mosquito bite. An apposite experimental system in which to delineate the pathogenesis of malaria is described in detail.
疟原虫的连续传代会增加其毒力，而蚊子传播则在哺乳动物宿主体内内在地调节寄生虫的毒力。因此，在研究疟疾的各个方面时，都必须考虑到完整的疟原虫生命周期。
间日疟原虫（Plasmodium chabaudi chabaudi）表现出许多与人类疟原虫感染自然蚊子媒介和哺乳动物宿主相关的特征，因此为在单次感染环境中研究疟疾的发病机制提供了独特的机会。我们开发了一种优化的方案，可通过按蚊（Anopheles stephensi）高效且可重复地传播 P. c. chabaudi。
该方案用于遗传上不同的 P. c. chabaudi 分离株的蚊子传播，突出了蚊子媒介内寄生虫毒力的差异，以及通过自然途径（蚊子叮咬）引发的感染中宿主易感性的范围。详细描述了一种适当的实验系统，用于描绘疟疾的发病机制。
