利用激光诱导铕离子发光光谱法通过与铕离子竞争来测定镁离子-ATP解离常数。

The determination of the Mg2+.ATP dissociation constant by competition with Eu3+ ion using laser-induced Eu3+ ion luminescence spectroscopy.

作者信息

McNemar C W, Horrocks W D

机构信息

Department of Chemistry, Pennsylvania State University, University Park 16802.

出版信息

Anal Biochem. 1990 Jan;184(1):35-8. doi: 10.1016/0003-2697(90)90007-v.

DOI:10.1016/0003-2697(90)90007-v

PMID:2321757

Abstract

A direct spectroscopic method for the determination of the submicromolar dissociation constant of Eu3+. ATP using laser-induced Eu3+ ion luminescence spectroscopy is described. The dissociation constant of Mg2+.ATP is then determined by the competition of Mg2+ with Eu3+ for the binding of ATP. The experiments were performed in 2H2O to mitigate the significant quenching of the Eu3+ luminescence that occurs in 1H2O. Values for the effective dissociation constants of the 1:1 ATP metal ion complexes of 1.2 +/- 0.3 X 10(-7) and 2.7 +/- 0.7 X 10(-4) M are obtained for Eu3+ and Mg2+, respectively, at p2H 5.8.

摘要

描述了一种使用激光诱导的Eu3+离子发光光谱法直接测定Eu3+.ATP亚微摩尔解离常数的光谱方法。然后通过Mg2+与Eu3+竞争ATP结合来测定Mg2+.ATP的解离常数。实验在2H2O中进行，以减轻在1H2O中发生的Eu3+发光的显著猝灭。在p2H 5.8时，Eu3+和Mg2+的1:1 ATP金属离子配合物的有效解离常数分别为1.2±0.3×10(-7)和2.7±0.7×10(-4) M。