一种用于测定1H2O/2H2O混合物同位素组成的光学方法：Eu3+发光寿命。

An optical method for the determination of the isotopic composition of 1H2O/2H2O mixtures: Eu3+ luminescence lifetimes.

作者信息

Cornwall E H, Horrocks W D

机构信息

Department of Chemistry, Pennsylvania State University, University Park 16802.

出版信息

Anal Biochem. 1988 Nov 15;175(1):59-62. doi: 10.1016/0003-2697(88)90360-0.

DOI:10.1016/0003-2697(88)90360-0

PMID:2854376

Abstract

A spectroscopic method employing pulsed dye laser instrumentation is described for the determination of the 1H2O/2H2O composition of aqueous solutions by the measurement of reciprocal excited state lifetimes of EuEDTA-. The reciprocal lifetimes, gamma-1, of the 1H2O/2H2O mixtures increase linearly with the mole fraction of 1H2O. For EuEDTA- the relationship between gamma-1 and the mole fraction, chi H, of 1H2O in 1H2O/2H2O mixtures is expressed by the equation chi H = 0.37 gamma-1-0.152, with a sensitivity in chi H of +/- 0.02. The reciprocal lifetimes are independent of pH in the range 5.1 to 10.5, changes in ionic strength, and the type of buffer used in EuEDTA- containing solutions.

摘要

描述了一种采用脉冲染料激光仪器的光谱方法，通过测量EuEDTA⁻的激发态寿命倒数来测定水溶液中¹H₂O/²H₂O的组成。¹H₂O/²H₂O混合物的寿命倒数γ⁻¹随¹H₂O的摩尔分数线性增加。对于EuEDTA⁻，γ⁻¹与¹H₂O/²H₂O混合物中¹H₂O的摩尔分数χH之间的关系由方程χH = 0.37γ⁻¹ - 0.152表示，χH的灵敏度为±0.02。寿命倒数在5.1至10.5的pH范围内、离子强度变化以及含EuEDTA⁻溶液中使用的缓冲液类型均无关。