Faculdade de Veterinária - Universidade Federal de Pelotas, Brazil.
Vet Parasitol. 2013 Mar 31;193(1-3):312-5. doi: 10.1016/j.vetpar.2012.11.012. Epub 2012 Nov 12.
The objective of this study was to assess the interleukin 10 (IL-10) concentrations in the sera of dogs suffering from demodicosis.
Twenty-six dogs were distributed into three groups: demodicosis groups G1, n=11 (recurring disease) and G2, n=6 (first time occurrence), and a control group, G3, n=9 (healthy dogs). All the animals were subjected to skin scrape tests and blood harvesting for serum extraction. In G1 and G2 only those animals with Demodex canis positive skin tests were included, while healthy dogs were included in G3. To assess IL-10 levels the commercial Quantikine Canine IL-10 Immunoassay(®) (R&D Systems) kit was used.
The mean IL-10 level obtained for G1 was 269.4 pg/ml (sd=290.8 pg/ml), for G2 it was 28.5 pg/ml (sd=19.7 pg/ml) while the mean for G3 was 11.9 pg/ml (sd=2.3 pg/ml). There was a significant difference between G1 and the other two groups.
According to our results, dogs with reoccurring demodicosis have higher IL-10 levels than healthy dogs and those suffering the disease for the first time.
本研究旨在评估患有蠕形螨病的犬血清中白细胞介素 10（IL-10）的浓度。
将 26 只犬分为三组：蠕形螨病组 G1，n=11（复发性疾病）和 G2，n=6（首次发病），以及对照组 G3，n=9（健康犬）。所有动物均进行皮肤刮片检查和采血以提取血清。仅在 G1 和 G2 中，那些皮肤试验阳性的犬被包括在内，而健康犬则被包括在 G3 中。为了评估 IL-10 水平，使用了商业 Quantikine 犬类 IL-10 免疫测定试剂盒（R&D Systems）。
G1 的平均 IL-10 水平为 269.4 pg/ml（sd=290.8 pg/ml），G2 为 28.5 pg/ml（sd=19.7 pg/ml），而 G3 的平均水平为 11.9 pg/ml（sd=2.3 pg/ml）。G1 与其他两组之间存在显著差异。
根据我们的结果，复发性蠕形螨病犬的 IL-10 水平高于健康犬和首次发病的犬。
