Gasparetto Naiani Domingos, Almeida Arleana do Bom Parto Ferreira, Nakazato Luciano, França Eduardo Luzía, França Adenilda Cristina Honorio, Fagundes Danny Laura Gomes, Bortolini Juliano, Sousa Valéria Régia Franco
Universidade Federal de Mato Grosso (UFMT), Programa de Pós Graduação em Ciências Veterinárias, Faculdade de Medicina Veterinária (FAVET), Cuiabá, Mato Grosso, Brazil.
Universidade Federal de Mato Grosso (UFMT), Faculdade de Medicina Veterinária (FAVET), Cuiabá, Mato Grosso, Brazil.
Vet Parasitol. 2018 Jun 15;257:1-4. doi: 10.1016/j.vetpar.2018.05.003. Epub 2018 May 7.
To quantify (by qPCR) the density of Demodex canis mites in the skin of dogs with demodicosis and in healthy dogs, as well as measuring the serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12, and tumour necrosis factor-alfa (TNF-α).
Fifty-four dogs were divided into three groups: localized demodicosis (LD, n = 16), generalized demodicosis (GD, n = 22), and control group (CG, n = 16). All dogs were subjected to skin scraping, blood collection, and skin biopsy. DNA extraction was performed and the parasite density was established by qPCR. Serum cytokine concentrations were obtained by flow cytometry.
The median number of mites in the skin of the GD (6.2 × 10 copies/μL) and LD dogs (1.2 × 10 copies/μL) was statistically higher than that in the CG dogs (8.7 × 10 copies/μL). Whereas there were no significant differences in median IL-1β, IL-8, IL-10, IL-12, and TNF-α levels among the study groups, there was a statistically higher IL-6 concentration in the LD dogs than in the healthy dogs.
According to our results, qPCR is an effective method for measuring the density of D. canis in the canine integument. In addition, the activation of the acute-phase immune response in localized demodicosis can be induced by IL-6 activity.
通过定量聚合酶链反应（qPCR）对患蠕形螨病犬和健康犬皮肤中犬蠕形螨的密度进行量化，并测定血清中白细胞介素（IL）-1β、IL-6、IL-8、IL-10、IL-12和肿瘤坏死因子-α（TNF-α）的浓度。
将54只犬分为三组：局限性蠕形螨病组（LD，n = 16）、全身性蠕形螨病组（GD，n = 22）和对照组（CG，n = 16）。所有犬均进行皮肤刮片、采血和皮肤活检。进行DNA提取，并通过qPCR确定寄生虫密度。通过流式细胞术获得血清细胞因子浓度。
GD组犬（6.2×10拷贝/μL）和LD组犬（1.2×10拷贝/μL）皮肤中的螨中位数在统计学上高于CG组犬（8.7×10拷贝/μL）。虽然研究组之间IL-1β、IL-8、IL-10、IL-12和TNF-α水平的中位数无显著差异，但LD组犬的IL-6浓度在统计学上高于健康犬。
根据我们的结果，qPCR是测量犬体表犬蠕形螨密度的有效方法。此外，局限性蠕形螨病中急性期免疫反应的激活可由IL-6活性诱导。
