College of Engineering, China Agricultural University, PO Box 50, 17 Qinghua Donglu, Beijing 100083, China.
Carbohydr Polym. 2013 Jan 30;92(1):98-105. doi: 10.1016/j.carbpol.2012.08.081. Epub 2012 Aug 30.
The viscoelastic property and scaling behavior of acid (glucono-δ-lactone)-induced soy protein isolate (SPI) gels were investigated at various ionic strengths (0-800mM) and five protein concentrations ranging between 4% and 8% (w/w). The infinite storage modulus ( [Formula: see text] ) and the gelation start time (t(g)) which indicate the progress of gelation process exhibited strong ionic strength dependence. The storage modulus and critical strain were found to exhibit a power-law relationship with protein concentration. Rheological analysis and confocal laser scanning microscopy (CLSM) analysis were applied to estimate the fractal dimensions (D(f)) of the gels and the values were found to vary between 2.319 and 2.729. The comparison of the rheological methods and the CLSM image analysis method showed that the Shih, Shih, Kim, Liu, and Aksay (1990) model was better suited in estimating the D(f) value of acid-induced SPI gel system.
研究了不同离子强度(0-800mM)和五种蛋白质浓度(4%-8%(w/w))下酸(葡庚糖内酯)诱导的大豆分离蛋白(SPI)凝胶的黏弹性和标度行为。无限储能模量([Formula: see text])和凝胶起始时间(t(g))表明凝胶过程的进展对离子强度有很强的依赖性。储存模量和临界应变与蛋白质浓度呈幂律关系。流变分析和共聚焦激光扫描显微镜(CLSM)分析用于估计凝胶的分形维数(D(f)),发现其值在 2.319 到 2.729 之间。流变方法和 CLSM 图像分析方法的比较表明,Shih、Shih、Kim、Liu 和 Aksay(1990)模型更适合于估计酸诱导的 SPI 凝胶体系的 D(f)值。