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硫酸钙诱导的大豆分离蛋白乳液凝胶中蛋白质聚集体的大小和含量对流变学及结构性质的影响

Effects of the size and content of protein aggregates on the rheological and structural properties of soy protein isolate emulsion gels induced by CaSO.

作者信息

Wang Xufeng, He Zhiyong, Zeng Maomao, Qin Fang, Adhikari Benu, Chen Jie

机构信息

State Key Laboratory of Food Science and Technology, and School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.

School of Applied Sciences, RMIT University, City Campus, Melbourne, VIC 3001, Australia.

出版信息

Food Chem. 2017 Apr 15;221:130-138. doi: 10.1016/j.foodchem.2016.10.019. Epub 2016 Oct 4.

Abstract

The effects of the size and content of soy protein isolate (SPI) aggregates on the rheological and textural properties of CaSO-induced SPI emulsion gels were investigated. Considerable differences in the rheological, water-holding, and micro-structural properties were observed. The gels with larger and/or more SPI aggregates showed substantial increase in the elastic modulus and had lower gelation temperatures. Creep data suggested that the size of the SPI aggregates contributed more to the elastic modulus, whereas the increase of aggregate content enhanced the elastic modulus and viscous component of the gels. The water-holding capacity was markedly enhanced (p<0.05) with the increase in both the size and content of SPI aggregates. Confocal laser scanning microscopy and scanning electron microscopy showed that larger and/or more SPI aggregates resulted in more homogeneous networks with smaller oil droplets. These insights provide important information for the product development in relation to soy protein-stabilized emulsions and emulsion gels.

摘要

研究了大豆分离蛋白(SPI)聚集体的大小和含量对硫酸钙诱导的SPI乳液凝胶流变学和质地特性的影响。观察到流变学、持水和微观结构特性存在显著差异。具有更大和/或更多SPI聚集体的凝胶显示出弹性模量大幅增加,且凝胶化温度较低。蠕变数据表明,SPI聚集体的大小对弹性模量的贡献更大,而聚集体含量的增加增强了凝胶的弹性模量和粘性成分。随着SPI聚集体大小和含量的增加,持水能力显著增强(p<0.05)。共聚焦激光扫描显微镜和扫描电子显微镜显示,更大和/或更多的SPI聚集体导致形成更均匀的网络,且油滴更小。这些见解为大豆蛋白稳定乳液和乳液凝胶的产品开发提供了重要信息。

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