具有强大趋化特性的羊膜间充质干细胞在治疗心肌梗死模型中是有效的。
Amniotic mesenchymal stem cells with robust chemotactic properties are effective in the treatment of a myocardial infarction model.
机构信息
Regional Clinical Trial Center, Dong-A University Hospital, Busan, Korea; Department of Cardiology, College of Medicine, Dong-A University, Busan, Korea.
出版信息
Int J Cardiol. 2013 Sep 30;168(2):1062-9. doi: 10.1016/j.ijcard.2012.11.003. Epub 2012 Dec 4.
BACKGROUND
We previously reported that amniotic mesenchymal stem cells (AMMs) possess high angio-vasulogenic properties. In this study, we investigated the chemotactic abilities of AMMs for improved cardiac function and regenerative angiogenesis.
METHODS
The expressions of chemotactic and angiogenic genes were determined by qRT-PCR. Myocardial infarction (MI) was induced in NOD/SCID mice and cells were directly transplanted into the border regions of ischemic heart tissue. Immunohistochemical analysis was also conducted.
RESULTS
AMMs significantly expressed the representative chemotactic factor GCP-2, NAP-2 as well as angiogenic factor Hif-1a. AMMs also highly expressed the chemokine receptors CCR2, CCR3 and CCR5. AMM transplantation improved left ventricular function, capillary density, angiogenic cytokine levels, angiopoetin (Ang)-1 and vascular endothelial growth factor (VEGF-A) levels in affected tissue. Immunohistochemical assaying also revealed increased engraftment and endothelial phenotypes.
CONCLUSION
Our findings suggest that due to elevated survival and related chemotactic potential, AMMs are a promising stem cell source for the treatment of ischemic cardiovascular disease.
背景
我们之前的研究表明，羊膜间充质干细胞（AMMs）具有较高的血管生成特性。在这项研究中，我们研究了 AMMs 的趋化能力，以改善心脏功能和再生血管生成。
方法
通过 qRT-PCR 确定趋化和血管生成基因的表达。在 NOD/SCID 小鼠中诱导心肌梗死（MI），并将细胞直接移植到缺血性心脏组织的边界区域。还进行了免疫组织化学分析。
结果
AMMs 显著表达了代表性趋化因子 GCP-2、NAP-2 以及血管生成因子 Hif-1a。AMMs 还高度表达趋化因子受体 CCR2、CCR3 和 CCR5。AMM 移植可改善左心室功能、毛细血管密度、促血管生成细胞因子水平、血管生成素（Ang）-1 和血管内皮生长因子（VEGF-A）水平。免疫组织化学检测还显示出增加的移植物和内皮表型。
结论
我们的研究结果表明，由于生存能力提高和相关趋化潜能增强，AMMs 是治疗缺血性心血管疾病的一种很有前途的干细胞来源。
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