Oncopathology Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
Pathol Res Pract. 2013 Feb 15;209(2):90-4. doi: 10.1016/j.prp.2012.10.010. Epub 2012 Dec 6.
Squamous cell carcinoma (SCC) is the second most common non-melanoma skin cancer. The oncogenic role of human papilloma virus (HPV) in cutaneous SCC has been suggested by several studies performed on immunosuppresed patients. However, the role of mucosal type HPV in SCC patients with normal immunity has not been studied extensively. Sixty skin biopsies from immunocompetent SCC patients and 60 benign skin specimens were evaluated for mucosal type HPV DNA using polymerase chain reaction (PCR) and immunohistochemistry (IHC). Mucosal type HPV DNA was detected in 18 of 60 cases (30%) and in 7 of 60 controls (11.6%) using PCR. HPV immunostain was positive in 16 of 60 cases (26.6%) and in 15 of 60 controls (25%). Mixed infection with HPV 18, 11, 6 was found in half of the SCC cases. The most prevalent subtype was HPV 18 followed by HPV 6 and 11. The frequency of HPV DNA was significantly elevated in our cases compared to controls (P value <0.01, OR=16.8, 95% CI: 3.3-74.9). Our findings suggest an association of mucosal type HPV, especially HPV 18, with skin SCC in Iranian patients with normal immunity.
鳞状细胞癌（SCC）是第二常见的非黑色素瘤皮肤癌。几项在免疫抑制患者中进行的研究表明，人乳头瘤病毒（HPV）在皮肤 SCC 中的致癌作用。然而，黏膜型 HPV 在免疫功能正常的 SCC 患者中的作用尚未得到广泛研究。使用聚合酶链反应（PCR）和免疫组织化学（IHC）评估了 60 例免疫功能正常的 SCC 患者和 60 例良性皮肤标本的黏膜型 HPV DNA。PCR 检测到 60 例中有 18 例（30%）和 60 例对照中有 7 例（11.6%）存在黏膜型 HPV DNA。HPV 免疫染色在 60 例中有 16 例（26.6%）和 60 例对照中有 15 例（25%）阳性。HPV 18、11、6 的混合感染在一半的 SCC 病例中发现。最常见的亚型是 HPV 18，其次是 HPV 6 和 11。与对照组相比，我们的病例中 HPV DNA 的频率显着升高（P 值<0.01，OR=16.8，95%CI：3.3-74.9）。我们的研究结果表明，黏膜型 HPV，尤其是 HPV 18，与伊朗免疫功能正常的皮肤 SCC 之间存在关联。
