Department of Obstetrics and Gynecology, Institute of Clinical Science, University of Gothenburg, Gothenburg, Sweden.
Fertil Steril. 2013 Mar 1;99(3):903-9. doi: 10.1016/j.fertnstert.2012.11.017. Epub 2012 Dec 7.
To characterize the size selectivity of the rat ovarian vasculature and its changes after gonadotropin induction of ovulation.
Experimental study.
Obstetrics and Gynecology Department.
ANIMAL(S): Immature, female Sprague-Dawley rats.
INTERVENTION(S): Rats were pretreated with equine chorionic gonadotropin, and ovaries were retrieved either 48 hours later or at any of several time points during ovulation induced by hCG. Fluorescein isothiocyanate-labeled Ficoll was injected 10 minutes before ovarian sampling, and the distribution of Ficoll was measured in plasma and ovarian extracts.
MAIN OUTCOME MEASURE(S): The Ficoll data were analyzed according to a two-pore model to acquire information on small (rS) and large (rL) pore radii as well as the number of large pores reflected by the large pore fraction of the hydraulic conductance (LpS%) at each periovulatory time interval.
RESULT(S): Before hCG, rS and rL were 54.7 ± 1.2 Å (mean ± SEM) and 149.3 ± 5.3 Å, respectively. At this preovulatory stage, LpS% was 7.1% ± 3.2%. Stimulation with hCG caused close to a three-fold increase in LpS% at 2 and 4 hours (20.9% ± 1.8% and 20.7% ± 2.5%, respectively) and approximately 15% enlargements of rS and rL. Thus, the change in LpS% represents a dramatic increase in the number of large pores and not an increased size of preexisting large pores, since the small and large pore radii changed in parallel.
CONCLUSION(S): These results indicate that capillary permeability of the ovarian blood-follicle barrier is modulated by gonadotropin, mainly through increased numbers of large pores, similar to a classical inflammatory response.
描述大鼠卵巢血管的大小选择性及其在促性腺激素诱导排卵后的变化。
实验研究。
妇产科。
未成熟雌性 Sprague-Dawley 大鼠。
大鼠先用马绒毛膜促性腺激素预处理，然后在 hCG 诱导排卵后 48 小时或排卵诱导过程中的任意几个时间点取出卵巢。在卵巢取样前 10 分钟注射异硫氰酸荧光素标记的 Ficoll，测量 Ficoll 在血浆和卵巢提取物中的分布。
根据双孔模型分析 Ficoll 数据，以获取小（rS）和大（rL）孔半径以及大孔分数反映的大孔数量（LpS%）的信息，每个排卵时间间隔。
在 hCG 之前，rS 和 rL 分别为 54.7±1.2Å（平均值±SEM）和 149.3±5.3Å。在这个排卵前阶段，LpS%为 7.1%±3.2%。用 hCG 刺激可使 2 小时和 4 小时时的 LpS%接近三倍增加（分别为 20.9%±1.8%和 20.7%±2.5%），并且 rS 和 rL 大约增加了 15%。因此，LpS%的变化代表大孔数量的急剧增加，而不是预先存在的大孔尺寸的增加，因为小和大孔半径是平行变化的。
这些结果表明，卵巢血管滤泡屏障的毛细血管通透性受促性腺激素调节，主要通过增加大孔的数量，类似于经典的炎症反应。
