Law M L, Chan S D, Berger R, Jones C, Kao F T, Solomon E, Cheah K S
Eleanor Roosevelt Institute for Cancer Research, University of Colorado, Health Sciences Center, Denver 80206.
Ann Hum Genet. 1990 Jan;54(1):23-9. doi: 10.1111/j.1469-1809.1990.tb00357.x.
A cosmid clone (CosHcol.11) containing the alpha 2(XI) collagen gene (COL11A2) has been isolated. The gene contains conserved DNA and amino-acid sequences characteristic of fibril forming collagen, which is in accordance with the classification of type XI collagen as a fibrillar collagen. The genomic clone containing the alpha 2(XI) gene has been used as probe in the Southern blot analysis of DNA from a panel of human/hamster somatic cell hybrids containing different numbers and combinations of human chromosomes. Synteny analysis revealed that only chromosome 6 showed complete concordant segregation with COL11A2. Furthermore, the gene was regionally mapped to the short arm of chromosome 6 by using a hybrid which contained only the long arm of the chromosome.
已分离出一个包含α2(XI)胶原基因（COL11A2）的黏粒克隆（CosHcol.11）。该基因含有形成纤维状胶原的保守DNA和氨基酸序列特征，这与XI型胶原作为纤维状胶原的分类一致。含有α2(XI)基因的基因组克隆已被用作探针，对一组包含不同数量和组合的人类染色体的人/仓鼠体细胞杂种的DNA进行Southern印迹分析。同线分析显示，只有6号染色体与COL11A2表现出完全一致的分离。此外，通过使用一个只包含该染色体长臂的杂种，该基因被定位到6号染色体的短臂上。
