Shows T B, Tikka L, Byers M G, Eddy R L, Haley L L, Henry W M, Prockop D J, Tryggvason K
Department of Human Genetics, Roswell Park Memorial Institute, Buffalo, New York 14263.
Genomics. 1989 Jul;5(1):128-33. doi: 10.1016/0888-7543(89)90096-7.
Type XIII collagen is a recently described collagen that resembles in structure the short-chain collagens of types IX, X, and XII. Unlike any other collagen, the type XIII is found in several different forms generated through alternative splicing. A 2.0-kb genomic fragment from the human alpha 1 (XIII) collagen gene was isolated and shown by DNA sequencing to contain exon 12 as counted from the 3' end. This fragment was used as a probe to localize the gene. The gene (COL13A1) was assigned to chromosome 10 by hybridization of the probe to DNA isolated from a panel of human-mouse somatic cell hybrids containing different human chromosomes. Furthermore, the gene was mapped to the q22 region by in situ hybridization to metaphase chromosomes.
ⅩⅢ型胶原是一种最近被描述的胶原，其结构类似于Ⅸ型、Ⅹ型和Ⅻ型短链胶原。与其他任何胶原不同，ⅩⅢ型胶原以几种通过可变剪接产生的不同形式存在。从人α1（ⅩⅢ）胶原基因中分离出一个2.0kb的基因组片段，并通过DNA测序显示其包含从3'端起计数的第12外显子。该片段用作探针来定位该基因。通过将该探针与从一组包含不同人类染色体的人-鼠体细胞杂种中分离的DNA杂交，将该基因（COL13A1）定位于10号染色体。此外，通过与中期染色体的原位杂交，将该基因定位到q22区域。
