Department of Histology and Embryology, Jessenius Faculty of Medicine Martin, Comenius University, Malá Hora 4, 03601 Martin, Slovakia.
Acta Histochem. 2013 Jun;115(5):412-7. doi: 10.1016/j.acthis.2012.10.001. Epub 2012 Dec 6.
The antiapoptotic protein survivin is rarely expressed in normal adult differentiated tissues, but it is often detected in their malignant counterparts. Immunohistochemically, we evaluated survivin expression in 19 cases of normal breast tissue and 64 cases of lobular breast carcinoma. The intensity of staining, percentage of labeled cells and subcellular location of survivin were assessed. We analyzed the quantitative differences of survivin expression between normal breast tissue and carcinomas. We also correlated survivin expression pattern in carcinomas with clinicomorphological parameters such as age of patients, grade, stage and size of primary tumor, lymph node metastasis, vascular invasion as well as estrogen and progesterone status. Survivin was detected in 10/19 cases of normal breast tissue (52.6%) and in 55/64 cases of lobular breast carcinoma (86%). The statistical analysis confirmed significant correlations between the assessed parameters in normal breast and lobular carcinoma. Furthermore, the expression of estrogen correlated significantly with the subcellular localization and intensity of survivin in carcinoma. However, no significant correlation was shown with regard to other clinicomorphological parameters. Our results suggest that survivin may be a valuable diagnostic marker, as well as a new independent prognostic parameter, in lobular breast carcinoma. Finally, our data support the hypothesis that lobular and ductal breast carcinomas seem to be different clinicomorphological entities.
凋亡抑制蛋白 survivin 在正常成年分化组织中很少表达，但在其恶性对应物中经常检测到。我们用免疫组织化学方法评估了 19 例正常乳腺组织和 64 例乳腺小叶癌中 survivin 的表达。评估了染色强度、标记细胞的百分比和 survivin 的亚细胞定位。我们分析了正常乳腺组织与癌之间 survivin 表达的定量差异。我们还将癌中 survivin 表达模式与患者年龄、分级、分期和肿瘤大小、淋巴结转移、血管浸润以及雌激素和孕激素状态等临床形态学参数相关联。在 19 例正常乳腺组织中有 10 例（52.6%）和 64 例乳腺小叶癌中有 55 例（86%）检测到 survivin。统计学分析证实了正常乳腺和乳腺小叶癌中评估参数之间的显著相关性。此外，雌激素的表达与癌中 survivin 的亚细胞定位和强度显著相关。然而，与其他临床形态学参数没有显示出显著相关性。我们的结果表明，survivin 可能是乳腺小叶癌的一个有价值的诊断标志物，也是一个新的独立预后参数。最后，我们的数据支持这样的假设，即乳腺小叶癌和导管癌似乎是不同的临床形态实体。
