运用闪光逆流色谱法从全反式视黄醛的异构化中分离出视网醇异构体。
Isolation of the retinal isomers from the isomerization of all-trans-retinal by flash countercurrent chromatography.
机构信息
Institute of Food Chemistry, Zhejiang Gongshang University, Hangzhou 3100125, China.
出版信息
J Chromatogr A. 2013 Jan 4;1271(1):67-70. doi: 10.1016/j.chroma.2012.11.022. Epub 2012 Nov 19.
The isolation of the retinal isomers from all-trans-retinal was performed by flash countercurrent chromatography. In each separation, isomerization reaction solution of 200mg all-trans-retinal could be loaded on a 1200 mL of high-speed countercurrent chromatographic column with 5mm bore, eluted by a mobile phase flow rate of 25 mL/min, resulting in 63 mg of 11-cis-retinal, 24 mg of 13-cis-retinal and 26 mg of 9-cis-retinal with purities more than 95%. n-Hexane-acetonitrile (3:1) was used as the solvent system which possesses the advantages of simplicity, re-use of the solvent and multiple injections. This method could be used to prepare 13-cis-retinal, 11-cis-retinal and 9-cis-retinal for the photoisomerization investigation, such as the effect of 11-cis-retinal in the visual system.
全反式视黄醛的立体异构体的分离是通过快速逆流色谱法实现的。在每次分离中，200mg 全反式视黄醛的异构化反应溶液可以加载到一个 1200 毫升的高速逆流色谱柱中，柱内径为 5 毫米，以 25 毫升/分钟的流动相流速洗脱，得到 63mg 的 11-顺式视黄醛、24mg 的 13-顺式视黄醛和 26mg 的 9-顺式视黄醛，纯度均超过 95%。正己烷-乙腈（3:1）被用作溶剂体系，该体系具有简单、溶剂可重复使用和多次进样的优点。该方法可用于制备 13-顺式视黄醛、11-顺式视黄醛和 9-顺式视黄醛，以进行光异构化研究，例如 11-顺式视黄醛在视觉系统中的作用。
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