Federal Institute for Risk Assessment (BfR), Department Chemicals Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
J Chromatogr A. 2013 Jan 4;1271(1):95-104. doi: 10.1016/j.chroma.2012.11.023. Epub 2012 Nov 19.
Based on the information available on 293 pesticides (herbicides, insecticides, fungicides, biocides, growth regulators) 210 pesticide metabolites were selected for inclusion into a multimethod for the analysis of ground and surface water. With the final method 150 pesticide metabolites can be analysed from groundwater and surface water by direct injection-liquid chromatography-electrospray ionization-tandem mass spectrometry with multiple-reaction monitoring. For most of these metabolites this is the first method published. For all metabolites linear calibration in drinking water was possible, with a lower limit of calibration of 0.1 μg/L achieved for 142 analytes and of 0.01 μg/L for 113 of the analytes. Matrix effects in ground and surface water compared to those in the drinking water were moderate (±20%) for 87% of the analytes. For critical sample/analyte combinations standard addition has to be used for correct quantification. This method allows for an extensive study of the occurrence of previously unknown or undetectable pesticide metabolites in groundwaters and surface waters.
基于 293 种农药（除草剂、杀虫剂、杀菌剂、杀生剂、生长调节剂）的信息，选择了 210 种农药代谢物，纳入多方法分析地下水和地表水。通过直接进样-液相色谱-电喷雾电离-串联质谱-多反应监测，最终方法可从地下水和地表水中分析 150 种农药代谢物。对于这些代谢物中的大多数，这是首次发表的方法。对于所有代谢物，在饮用水中都可以进行线性校准，其中 142 种分析物的校准下限为 0.1μg/L，113 种分析物的校准下限为 0.01μg/L。与饮用水相比，地下水和地表水中的基质效应对于 87%的分析物为中等（±20%）。对于关键的样品/分析物组合，必须使用标准添加来进行正确的定量。该方法可广泛研究地下水和地表水中以前未知或无法检测到的农药代谢物的出现情况。
