INSERM U866, Physiologie de la Nutrition & Toxicologie (NuTox), Université de Bourgogne, Dijon, France.
Int Immunopharmacol. 2013 Feb;15(2):364-71. doi: 10.1016/j.intimp.2012.11.013. Epub 2012 Dec 4.
We assessed the effects of Zizyphus lotus L. (Desf.) polyphenols (ZLP) on T-cell signaling and proliferation. Our results showed that ZLP exerted no effect on the increases in intracellular free calcium concentrations, [Ca(2+)]i, in human Jurkat T-cells. However, ZLP modulated the thapsigargin-induced increases in [Ca(2+)]i in these cells. ZLP treatment was found to decrease the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). In addition, ZLP induced a rapid (t1/2=33s) and dose-dependent decrease in intracellular pH (pHi) in human Jurkat T-cells. Furthermore, ZLP significantly curtailed T-cell proliferation by diminishing their progression from S to G2/M phase of cell cycle, and the expression of interleukin-2 (IL-2) mRNA. Taken together, the results of the present study demonstrate that ZLP modulate cell signaling and exert immunosuppressive effects in human T-cells.
我们评估了荷叶多酚（ZLP）对 T 细胞信号转导和增殖的影响。结果表明，ZLP 对人 Jurkat T 细胞内游离钙浓度 ([Ca(2+)]i) 的增加没有影响。然而，ZLP 调节了这些细胞中海藻糖诱导的 [Ca(2+)]i 增加。ZLP 处理可降低细胞外信号调节激酶 1/2（ERK1/2）的磷酸化。此外，ZLP 诱导人 Jurkat T 细胞内 pH 值（pHi）快速（t1/2=33s）和剂量依赖性下降。此外，ZLP 通过减少 T 细胞从 S 期到 G2/M 期的细胞周期进展，以及白细胞介素-2（IL-2）mRNA 的表达，显著抑制 T 细胞增殖。综上所述，本研究结果表明，ZLP 调节细胞信号转导，并在人 T 细胞中发挥免疫抑制作用。
