Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
J Virol Methods. 2013 Mar;188(1-2):6-12. doi: 10.1016/j.jviromet.2012.11.030. Epub 2012 Dec 5.
A new, reliable and secure virus assay method, named the competitive virus assay (CVA) method, has been established for the titration of bovine viral diarrhea viruses (BVDVs) that either show the exaltation of Newcastle disease virus (END) phenomenon or heterologous interference phenomenon (but not the END phenomenon). This method is based on the principle of (1) homologous interference between BVDVs, by using BVDV RK13/E(-) or BVDV RK13/E(+) strains as competitor virus, and (2) END phenomenon and heterologous interference, by using attenuated Newcastle disease virus (NDV) TCND strain as challenge virus. In titration of BVDV END(+) and BVDV END(-) viruses, no significant difference in estimated virus titer was observed between CVA and conventional methods. CVA method demonstrated comparable levels of sensitivity and accuracy as conventional END and interference methods, which require the use of a velogenic Miyadera strain of NDV and vesicular stomatitis virus (VSV), both of which are agents of high-risk diseases. As such, the CVA method is a safer alternative, with increased bio-safety and bio-containment, through avoidance of virulent strains that are commonly employed with conventional methods.
一种新的、可靠的和安全的病毒检测方法，称为竞争病毒检测（CVA）方法，已经建立用于滴定牛病毒性腹泻病毒（BVDV），这些病毒要么表现出新城疫病毒（END）现象的增强，要么表现出异源干扰现象（但不是 END 现象）。该方法基于以下原理：（1）BVDV 之间的同源干扰，使用 BVDV RK13/E(-)或 BVDV RK13/E(+)株作为竞争病毒，以及（2）END 现象和异源干扰，使用弱毒新城疫病毒（NDV）TCND 株作为攻击病毒。在滴定 BVDV END(+)和 BVDV END(-)病毒时，CVA 和常规方法估计的病毒滴度之间没有显著差异。CVA 方法与传统的 END 和干扰方法具有可比的灵敏度和准确性，这些方法需要使用强毒 Miyadera 株新城疫病毒和水疱性口炎病毒（VSV），这两种病毒都是高风险疾病的病原体。因此，CVA 方法是一种更安全的替代方法，通过避免传统方法中常用的强毒株，提高了生物安全性和生物遏制性。
