Rossi C R, Kiesel G K
Am J Vet Res. 1983 Oct;44(10):1916-9.
The use of polyriboinosinic acid:polyribocytidylic acid (poly I:C) for noncytopathogenic bovine viral diarrhea virus (NC-BVDV) assay (PINBA) was studied. Several viruses were tested for their suitability as test challenge viruses. In addition to vesicular stomatitis virus, which previously was shown to be a suitable challenge virus, bovine enteroviruses also were found to be suitable, whereas infectious bovine rhinotracheitis virus and parainfluenza type 3 virus were only marginally suitable. Bovine embryonic skin (BES) cultures developed resistance to PINBA within a few in vitro passages, whereas bovine embryonic lung (BEL) cultures did not. At certain passages, BES cultures were 500,000 times more resistant than BEL cultures. To determine whether the difference in viral titers on BEL and BES cultures was due to NC-BVDV replication, PINBA and fluorescent antibody assays were compared on the cultures. Resistance of BES cultures to PINBA was not due to an inability of the virus to replicate in the cultures, but was due to an inability of PINBA to detect the virus. Viral titers were comparable by fluorescent antibody assay titers on BES and BEL cultures, but were considerably higher than viral titers on BES cultures with PINBA. Variations in viral titers, using PINBA on BEL cultures, were observed and were considered to be due to cultural conditions, such as the presence of low levels of BVDV antibodies in bovine fetal serum used in the medium. Treatment of BEL cultures with poly I:C or interferon showed that NC-BVDV was sensitive to interferon as determined by virus-yield reduction.
聚胞苷酸（poly I:C）在非致细胞病变性牛病毒性腹泻病毒（NC-BVDV）检测（PINBA）中的应用。测试了几种病毒作为试验攻击病毒的适用性。除了之前已证明是合适攻击病毒的水疱性口炎病毒外，还发现牛肠道病毒也合适，而传染性牛鼻气管炎病毒和3型副流感病毒仅勉强合适。牛胚胎皮肤（BES）培养物在体外传代几次后对PINBA产生抗性，而牛胚胎肺（BEL）培养物则没有。在某些传代时，BES培养物的抗性比BEL培养物高500,000倍。为了确定BEL和BES培养物上病毒滴度的差异是否归因于NC-BVDV复制，在这些培养物上比较了PINBA和荧光抗体检测。BES培养物对PINBA的抗性不是由于病毒无法在培养物中复制，而是由于PINBA无法检测到病毒。通过荧光抗体检测，BES和BEL培养物上的病毒滴度相当，但比PINBA检测的BES培养物上的病毒滴度高得多。观察到在BEL培养物上使用PINBA时病毒滴度的变化，认为这归因于培养条件，例如培养基中使用的牛胎血清中存在低水平的BVDV抗体。用poly I:C或干扰素处理BEL培养物表明，通过病毒产量降低测定，NC-BVDV对干扰素敏感。
