Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Biochem Biophys Res Commun. 2013 Jan 11;430(2):598-603. doi: 10.1016/j.bbrc.2012.11.107. Epub 2012 Dec 5.
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the immunoglobulin super family and has been observed to have two paradoxical functions: tumor suppression and the promotion of tumor invasion. In the present study, we discovered that CEACAM1 functions as an adhesion inhibitor and a migration promoter. The CEACAM1 transfected cells, either 293-CEACAM1 or LOVO/trans-CEACAM1, was proved to have lower adhesion rate. Furthermore, HT29/siRNA-CEACAM1 cells had a higher adhesion rate than HT29 cells. These results indicated that CEACAM1 was an inhibitor of cell-matrix adhesion. Additionally, 293-CEACAM1 LOVO/trans-CEACAM1 cells exhibited better motility in a trans-well migration assay. N-cadherin expression levels were positively correlated with CEACAM1 in 293-CEACAM1, LOVO/trans-CEACAM1 and HT29/siRNA-CEACAM1 cells. When blocked by a GC-4 antibody, the adhesive capacities of 293-CEACAM1 and LOVO/trans-CEACAM1 were recovered and the motilities of them were suppressed, which suggested that CEACAM1 functioned through N-cadherin.
癌胚抗原相关细胞黏附分子 1（CEACAM1）是免疫球蛋白超家族的成员，具有两种相互矛盾的功能：肿瘤抑制和促进肿瘤侵袭。在本研究中，我们发现 CEACAM1 作为黏附抑制剂和迁移促进剂发挥作用。转染 CEACAM1 的 293-CEACAM1 或 LOVO/trans-CEACAM1 细胞被证实具有较低的黏附率。此外，HT29/siRNA-CEACAM1 细胞的黏附率高于 HT29 细胞。这些结果表明 CEACAM1 是细胞-基质黏附的抑制剂。此外，293-CEACAM1、LOVO/trans-CEACAM1 细胞在 Transwell 迁移实验中表现出更好的迁移能力。在 293-CEACAM1、LOVO/trans-CEACAM1 和 HT29/siRNA-CEACAM1 细胞中，N-钙黏蛋白表达水平与 CEACAM1 呈正相关。当被 GC-4 抗体阻断时，293-CEACAM1 和 LOVO/trans-CEACAM1 的黏附能力得到恢复，其迁移能力受到抑制，这表明 CEACAM1 通过 N-钙黏蛋白发挥作用。
