CEACAM1 通过调节 N-钙黏蛋白的表达抑制细胞-基质黏附并促进细胞迁移。
CEACAM1 inhibits cell-matrix adhesion and promotes cell migration through regulating the expression of N-cadherin.
机构信息
Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
出版信息
Biochem Biophys Res Commun. 2013 Jan 11;430(2):598-603. doi: 10.1016/j.bbrc.2012.11.107. Epub 2012 Dec 5.
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the immunoglobulin super family and has been observed to have two paradoxical functions: tumor suppression and the promotion of tumor invasion. In the present study, we discovered that CEACAM1 functions as an adhesion inhibitor and a migration promoter. The CEACAM1 transfected cells, either 293-CEACAM1 or LOVO/trans-CEACAM1, was proved to have lower adhesion rate. Furthermore, HT29/siRNA-CEACAM1 cells had a higher adhesion rate than HT29 cells. These results indicated that CEACAM1 was an inhibitor of cell-matrix adhesion. Additionally, 293-CEACAM1 LOVO/trans-CEACAM1 cells exhibited better motility in a trans-well migration assay. N-cadherin expression levels were positively correlated with CEACAM1 in 293-CEACAM1, LOVO/trans-CEACAM1 and HT29/siRNA-CEACAM1 cells. When blocked by a GC-4 antibody, the adhesive capacities of 293-CEACAM1 and LOVO/trans-CEACAM1 were recovered and the motilities of them were suppressed, which suggested that CEACAM1 functioned through N-cadherin.
癌胚抗原相关细胞黏附分子 1（CEACAM1）是免疫球蛋白超家族的成员，具有两种相互矛盾的功能：肿瘤抑制和促进肿瘤侵袭。在本研究中，我们发现 CEACAM1 作为黏附抑制剂和迁移促进剂发挥作用。转染 CEACAM1 的 293-CEACAM1 或 LOVO/trans-CEACAM1 细胞被证实具有较低的黏附率。此外，HT29/siRNA-CEACAM1 细胞的黏附率高于 HT29 细胞。这些结果表明 CEACAM1 是细胞-基质黏附的抑制剂。此外，293-CEACAM1、LOVO/trans-CEACAM1 细胞在 Transwell 迁移实验中表现出更好的迁移能力。在 293-CEACAM1、LOVO/trans-CEACAM1 和 HT29/siRNA-CEACAM1 细胞中，N-钙黏蛋白表达水平与 CEACAM1 呈正相关。当被 GC-4 抗体阻断时，293-CEACAM1 和 LOVO/trans-CEACAM1 的黏附能力得到恢复，其迁移能力受到抑制，这表明 CEACAM1 通过 N-钙黏蛋白发挥作用。
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