Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Respir Physiol Neurobiol. 2013 Feb 1;185(3):608-14. doi: 10.1016/j.resp.2012.11.016. Epub 2012 Dec 6.
Resveratrol is a phytoalexin synthesized by a wide variety of plants, which has been proven to be effective in suppressing oxidative stress and inflammation. The aim of the present study was to investigate the effect of Resveratrol's prodrug: 3,5,4'-tri-O-acetylresveratrol, on seawater drowning-induced acute lung injury (SWD-ALI). Histological changes were assessed to study lung injuries; cytokines in lung samples were monitored by ELISA to reflect inflammation; T-SOD and MDA activity were detected to examine oxidative stress in lung tissues. Besides, we also tested the expression of NF-κB and HIF-1α to probe the possible protecting mechanism of 3,5,4'-tri-O-acetylresveratrol on AWD-ALI. The results showed that pretreatment with different doses of 3,5,4'-tri-O-acetylresveratrol improved seawater-induced lung histopathologic changes, alleviated lung edema, reduced the production of inflammatory mediators including TNF-α and IL-1β, inhibited MDA activity, and enhanced T-SOD activity, which was possibly associated with inhibition of NF-κB and HIF-1α. In conclusion, the current study demonstrated that 3,5,4'-tri-O-acetylresveratrol exhibited a protective effect on SWD-ALI by inhibiting of the inflammatory response, which may also involve the suppression of oxidative stress in lung tissues.
白藜芦醇是一种广泛存在于植物中的植物抗毒素，已被证明可有效抑制氧化应激和炎症。本研究旨在探讨白藜芦醇前药：3,5,4'-三-O-乙酰白藜芦醇对海水溺水致急性肺损伤（SWD-ALI）的影响。通过评估组织学变化来研究肺损伤；通过 ELISA 监测肺样本中的细胞因子以反映炎症；检测 T-SOD 和 MDA 活性以检查肺组织中的氧化应激。此外，我们还检测了 NF-κB 和 HIF-1α 的表达，以探究 3,5,4'-三-O-乙酰白藜芦醇对 AWD-ALI 的可能保护机制。结果表明，用不同剂量的 3,5,4'-三-O-乙酰白藜芦醇预处理可改善海水诱导的肺组织病理变化，减轻肺水肿，减少 TNF-α和 IL-1β等炎症介质的产生，抑制 MDA 活性，增强 T-SOD 活性，这可能与抑制 NF-κB 和 HIF-1α有关。总之，本研究表明 3,5,4'-三-O-乙酰白藜芦醇通过抑制炎症反应对 SWD-ALI 具有保护作用，这可能还涉及抑制肺组织中的氧化应激。
