Gould S A, Sehgal L R, Rosen A L, Sehgal H L, Moss G S
Department of Surgery, Michael Reese Hospital, Chicago, IL 60616.
Ann Surg. 1990 Apr;211(4):394-8. doi: 10.1097/00000658-199004000-00003.
A polymerized pyridoxylated hemoglobin solution (Poly SFH-P) has been prepared with a normal [Hb] of 14 g/dL, a normal COP of 20 to 25 torr, a P50 of 16 to 20 torr, and a plasma T1/2 of 40 to 46 hours. Animals underwent a total exchange transfusion with Poly SFH-P to assess its ability to support hemodynamics and oxygen transport in the absence of red cells. All animals survived the exchange transfusion. Mean arterial pressure (MAP), heart rate (HR), cardiac output (CO), and oxygen consumption (VO2) remained at baseline values at zero hematocrit after the exchange. The final plasma [Hb] at Hct less than 1% was 9.7 +/- 0.4 g/dL. These results are significantly better than previous data with unmodified tetrameric hemoglobin solution (SFH). Poly SFH-P supports life in the absence of red cells. In contrast to SFH, Poly SFH-P achieves a nearly normal [Hb], a longer T1/2, and maintains baseline hemodynamics and oxygen consumption at zero hematocrit. These results document that Poly SFH-P is an effective oxygen carrier that offers greater potential than previous products as a clinically useful red cell substitute.
已制备出一种聚合的吡哆醛化血红蛋白溶液（Poly SFH-P），其血红蛋白（Hb）含量正常为14 g/dL，胶体渗透压（COP）正常为20至25托，P50为16至20托，血浆半衰期（T1/2）为40至46小时。对动物进行了用Poly SFH-P的全血交换输血，以评估其在无红细胞情况下支持血流动力学和氧运输的能力。所有动物在交换输血后存活。交换后血细胞比容为零时，平均动脉压（MAP）、心率（HR）、心输出量（CO）和氧耗量（VO2）保持在基线值。血细胞比容小于1%时的最终血浆Hb含量为9.7±0.4 g/dL。这些结果明显优于先前未修饰的四聚体血红蛋白溶液（SFH）的数据。Poly SFH-P在无红细胞情况下可维持生命。与SFH不同，Poly SFH-P能达到近乎正常的Hb含量、更长的T1/2，并在血细胞比容为零时维持基线血流动力学和氧耗量。这些结果证明，Poly SFH-P是一种有效的氧载体，作为一种临床上有用的红细胞替代物，其潜力比先前的产品更大。
