Department of Clinical Microbiology, Skåne University Hospital, Malmö, Sweden.
Cancer Epidemiol Biomarkers Prev. 2013 Feb;22(2):242-50. doi: 10.1158/1055-9965.EPI-12-1003. Epub 2012 Dec 5.
There is a need for a rapid and cost-effective evaluation of the effects of different human papillomavirus (HPV) vaccination strategies. Sexually active adolescents are a preferred target group for monitoring, as effects on HPV prevalence would be measurable shortly after implementation of vaccination programs.
The Swedish Chlamydia trachomatis testing program offers free Chlamydia trachomatis testing and reaches a majority of all adolescents in the population. We anonymized the 44,146 samples submitted for Chlamydia trachomatis testing in Southern Sweden during March to November 2008 and conducted HPV genotyping using PCR followed by mass spectrometry.
The HPV positivity peaked at 54.4% [95% confidence interval (CI), 52.2-56.6] among 21-year-old women and at 15.0% (95% CI, 12.4-17.6) among 23-year-old men. The HPV positivity was 37.8% (95% CI, 37.3-38.3) for women and 11.2% (95% CI, 10.6-11.8) for men. The most prevalent types among women were HPV 16 (10.0%; 95% CI, 9.7-10.3) and HPV 51 (6.0%; 95% CI, 5.7-6.3) and, among men, HPV 16 (2.1%; 95% CI, 1.8-2.4) and HPV 6 and HPV 51 (1.7%; 95% CI, 1.5-1.9).
The high HPV prevalences seen in the Chlamydia trachomatis screening population enables monitoring of the HPV type distribution among sexually active adolescents at high precision.
Effectiveness of HPV vaccination programs in terms of reducing HPV infections has been difficult to measure because of logistic constraints. We describe a system for high-throughput monitoring of HPV type-specific prevalences using samples from the Chlamydia trachomatis screening program.
需要快速且具有成本效益的方法来评估不同人乳头瘤病毒（HPV）疫苗接种策略的效果。性活跃的青少年是监测的首选目标人群，因为在接种疫苗计划实施后不久，HPV 流行率的影响就可以测量到。
瑞典的衣原体检测计划提供免费的衣原体检测，可覆盖人群中大多数青少年。我们对 2008 年 3 月至 11 月在瑞典南部提交的用于衣原体检测的 44146 个样本进行了匿名处理，并使用聚合酶链反应（PCR）和质谱法进行 HPV 基因分型。
21 岁女性 HPV 阳性率最高，为 54.4%（95%置信区间[CI]，52.2-56.6），23 岁男性 HPV 阳性率最低，为 15.0%（95%CI，12.4-17.6）。女性 HPV 阳性率为 37.8%（95%CI，37.3-38.3），男性 HPV 阳性率为 11.2%（95%CI，10.6-11.8）。女性最常见的 HPV 类型是 HPV 16（10.0%；95%CI，9.7-10.3）和 HPV 51（6.0%；95%CI，5.7-6.3），而男性最常见的 HPV 类型是 HPV 16（2.1%；95%CI，1.8-2.4）和 HPV 6 和 HPV 51（1.7%；95%CI，1.5-1.9）。
在衣原体筛查人群中观察到的高 HPV 流行率使我们能够以高精度监测性活跃青少年的 HPV 型别分布。
由于后勤方面的限制，HPV 疫苗接种计划在降低 HPV 感染方面的效果一直难以衡量。我们描述了一种使用衣原体筛查计划样本进行 HPV 型特异性流行率高通量监测的系统。
