Kim Mi-Sun, Kim HyunSoon, Moon Jae Sun, Hwang Ingyu, Joung Hyouk, Jeon Jae-Heung
Green-Bio Research Center, KRIBB, Daejeon, Korea.
Biosci Biotechnol Biochem. 2012;76(12):2354-6. doi: 10.1271/bbb.120593. Epub 2012 Dec 7.
This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.
本研究建立了一种新的马铃薯转化系统,该系统使用毒黄素作为选择剂,以毒黄素裂解酶(tflA)作为选择标记基因。表达tflA的马铃薯植株在毒黄素培养基上成功转化,转化效率为27%,与潮霉素/hpt选择系统的效率相似。表达tflA的转基因马铃薯也表现出对水稻细菌性条斑病菌感染的抗性。