Chemical synthesis of bovine transforming growth factor-alpha: synthesis, characterization and biological activity.
作者信息
Tou J S, McGrath M F, Zupec M E, Byatt J C, Violand B N, Kaempfe L A, Vineyard B D
机构信息
Monsanto Company, St. Louis, Missouri 63198.
出版信息
Biochem Biophys Res Commun. 1990 Mar 16;167(2):484-91. doi: 10.1016/0006-291x(90)92049-6.
Bovine transforming growth factor-alpha (bTGF-alpha) is a 50 amino acid polypeptide with three disulfide linkages. In order to evaluate the biological function of this peptide, bTGF-alpha was synthesized via an automatic synthesizer and purified to homogeneity in high yield. The integrity of this synthetic peptide was confirmed by chemical analyses and bioassays. In a bovine liver radioreceptor assay, bTGF-alpha competes with radiolabeled EGF and has activity comparable to mEGF and hTGF-alpha. Compared to hEGF, bTGF-alpha elicits a greater response in a bovine mammary cell proliferation.
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