Krajewska-Grynkiewicz K, Kłopotowski T
Mol Gen Genet. 1979 Oct 2;176(1):87-93. doi: 10.1007/BF00334299.
The effects of distant deletions or insertions in the Salmonella typhimurium donor strains on P22--mediated cotransducibility of genetic markers was studied. We found that deletions of histidine operon, unit 44 of the chromosome map, changed the linkage of markers purF and aroC (unit 49) and pyrF and trpA (unit 34). They did not change the linkage of more distant markers pyrE and cysE. The effect of three types of insertions was examined. The donor strains carried F factor, Tn10 transposon or pi-his duplication inserted close to histidine operon. These insertions caused alteration of purF-aroC linkage while pyrF-trpA cotransduction values were not affected. These data show that the effect of the chromosome rearrangements extends to at least 5% of S. typhimurium chromosome length and may reach as much as 10% of it. Our results are in agreement with the model of Chelala and Margolin (1974) concerning formation of transduction particles. They indicate that the cotransducibility changes caused by deletions or insertions extent further than it might have been expected from previous reports.
研究了鼠伤寒沙门氏菌供体菌株中远距离缺失或插入对P22介导的遗传标记共转导性的影响。我们发现，组氨酸操纵子（染色体图谱的第44单元）的缺失改变了标记purF和aroC（第49单元）以及pyrF和trpA（第34单元）的连锁关系。它们并未改变更远距离标记pyrE和cysE的连锁关系。研究了三种类型插入的影响。供体菌株携带插入在组氨酸操纵子附近的F因子、Tn10转座子或pi-组氨酸重复序列。这些插入导致purF-aroC连锁关系改变，而pyrF-trpA共转导值未受影响。这些数据表明，染色体重排的影响至少延伸到鼠伤寒沙门氏菌染色体长度的5%，可能达到其10%。我们的结果与Chelala和Margolin（1974年）关于转导颗粒形成的模型一致。它们表明，由缺失或插入引起的共转导性变化比以前报道预期的范围更广。
