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关于噬菌体P22 DNA的连续包装

On the sequential packaging of bacteriophage P22 DNA.

作者信息

Adams M B, Hayden M, Casjens S

出版信息

J Virol. 1983 May;46(2):673-7. doi: 10.1128/JVI.46.2.673-677.1983.

Abstract

Bacteriophage P22 is thought to package daughter chromosomes serially along concatemeric DNA. We present experiments which show that the average DNA packaging series length increases with time after infection, which supports this model. In addition, we have analyzed the effect on average series length of lowering the amount of the various individual proteins involved in DNA packaging. These results support the notion that the protein products of gene 2 and gene 3 are both more stringently required for initiation of sequential DNA packaging series than for their extension, and they are compatible with a model for the control of series length in which that length is determined, at least in part, by a competition between series initiation events and extension events.

摘要

噬菌体P22被认为是沿着串联DNA依次包装子代染色体。我们进行的实验表明,感染后平均DNA包装序列长度随时间增加,这支持了该模型。此外,我们分析了降低参与DNA包装的各种单个蛋白质的量对平均序列长度的影响。这些结果支持以下观点:与DNA包装序列延伸相比,基因2和基因3的蛋白质产物对连续DNA包装序列起始的严格要求更高,并且它们与序列长度控制模型相符,在该模型中,序列长度至少部分由序列起始事件和延伸事件之间的竞争决定。

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Genetics of bacteriophage P22. III. The late operon.
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