Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978, Israel.
J Biomed Opt. 2012 Oct;17(10):101509. doi: 10.1117/1.JBO.17.10.101509.
We propose a low-coherence spectral-domain phase microscopy (SDPM) system for accurate quantitative phase measurements in red blood cells (RBCs) for the prognosis and monitoring of disease conditions that affect the visco-elastic properties of RBCs. Using the system, we performed time-recordings of cell membrane fluctuations, and compared the nano-scale fluctuation dynamics of healthy and glutaraldehyde-treated RBCs. Glutaraldehyde-treated RBCs possess lower amplitudes of fluctuations, reflecting an increased membrane stiffness. To demonstrate the ability of our system to measure fluctuations of lower amplitudes than those measured by the commonly used holographic phase microscopy techniques, we also constructed wide-field digital interferometry (WFDI) system and compared the performances of both systems. Due to its common-path geometry, the optical-path-delay stability of SDPM was found to be less than 0.3 nm in liquid environment, at least three times better than WFDI under the same conditions. In addition, due to the compactness of SDPM and its inexpensive and robust design, the system possesses a high potential for clinical applications.
我们提出了一种低相干光谱域相显微镜（SDPM）系统，用于对红细胞（RBC）进行精确的定量相位测量，以便对影响 RBC 粘弹性的疾病状况进行预后和监测。使用该系统，我们对细胞膜波动进行了时间记录，并比较了健康和戊二醛处理的 RBC 的纳米级波动动力学。戊二醛处理的 RBC 具有较低的波动幅度，反映出膜刚性增加。为了证明我们的系统能够测量比常用全息相位显微镜技术测量的波动幅度更低的波动，我们还构建了宽场数字干涉（WFDI）系统，并比较了这两种系统的性能。由于其共路几何结构，在液体环境中，SDPM 的光程延迟稳定性被发现小于 0.3nm，至少比 WFDI 在相同条件下好三倍。此外，由于 SDPM 的紧凑性及其廉价和坚固的设计，该系统具有很高的临床应用潜力。
