Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, via G. Balzaretti 9, 20133 Milan, Italy.
Amino Acids. 2013 Mar;44(3):1001-8. doi: 10.1007/s00726-012-1433-5. Epub 2012 Dec 6.
To investigate the influence of diet on serum protein pattern, mice were fed for 8 weeks either control chow or a high-fat diet (containing 21 % w/w milk fat and 0.2 % w/w cholesterol); sera were collected and analyzed by 2-DE. The main positive acute-phase reactant proteins, haptoglobin and hemopexin, were significantly up-regulated in animals receiving the high-fat diet. Data on all other proteins also pointed to an inflammatory condition in these animals. The largest change in concentration was observed for carboxylesterase N, a circulating enzyme seldom connected with lipid metabolism in earlier reports. These observations agree with the notion of a link between diet-induced hyperlipidemia and the inflammatory component of its cardiovascular sequels in humans, but the effects in the experimental animals are massive and obviously affect most of the major serum proteins.
为了研究饮食对血清蛋白图谱的影响，将小鼠分别用对照饲料或高脂肪饲料（含 21%的乳脂肪和 0.2%的胆固醇）喂养 8 周；收集血清并通过二维电泳进行分析。主要的急性反应期阳性反应物蛋白，触珠蛋白和血红素结合蛋白，在接受高脂肪饮食的动物中明显上调。所有其他蛋白的数据也表明这些动物存在炎症状态。浓度变化最大的是羧基酯酶 N，这是一种在早期报告中很少与脂代谢有关的循环酶。这些观察结果与饮食引起的高脂血症与人类心血管并发症的炎症成分之间存在联系的观点一致，但实验动物的影响是巨大的，显然会影响大多数主要的血清蛋白。
