Chang Jing-Xia, Gao Feng, Zhao Guo-Qiang, Zhang Guo-Jun
Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University;
Exp Ther Med. 2012 Nov;4(5):795-800. doi: 10.3892/etm.2012.693. Epub 2012 Sep 3.
Treatment failure for lung adenocarcinoma is frequently due to lymph node metastasis and invasion to neighboring organs. The aim of the present study was to investigate the invasion- and metastasis-related gene, neural precursor cell expressed, developmentally downregulated 9 (NEDD9), in lung adenocarcinoma tissues and cell lines. The expression of NEDD9 was analyzed by the SP method of immunohistochemistry for 60 formalin-fixed and paraffin-embedded (FFPE) lung adenocarcinoma tissues in which 32 cases were metastastic and 28 were without metastases. NEDD9 mRNA expression and protein levels were quantified by fluorescence quantitative reverse transcription-polymerase chain reaction (FQ-PCR) and western blotting in the highly invasive lung adenocarcinoma cell lines A549 and 95D as well as in SPC-A-1 cells with low invasive potential. The immunostaining scores revealed a statistically significant difference between metastatic and non-metastatic lung adenocarcinomas (p<0.001). FQ-PCR and western blotting demonstrated that NEDD9 expression was higher in A549 and 95D compared to SPC-A-1 cells (P=0.003). Our results provide evidence that NEDD9 is upregulated in metastatic lung adenocarcinoma and in highly invasive lung adenocarcinoma cell lines, suggesting its potential involvement in regulating cell migration and invasion.
肺腺癌的治疗失败通常是由于淋巴结转移和邻近器官浸润。本研究旨在调查肺腺癌组织和细胞系中与侵袭和转移相关的基因——神经前体细胞表达、发育下调基因9（NEDD9）。采用免疫组织化学SP法分析60例福尔马林固定石蜡包埋（FFPE）肺腺癌组织中NEDD9的表达，其中32例发生转移，28例未发生转移。采用荧光定量逆转录聚合酶链反应（FQ-PCR）和蛋白质印迹法检测高侵袭性肺腺癌细胞系A549和95D以及低侵袭潜能的SPC-A-1细胞中NEDD9 mRNA表达和蛋白水平。免疫染色评分显示，转移性和非转移性肺腺癌之间存在统计学显著差异（p<0.001）。FQ-PCR和蛋白质印迹法表明，与SPC-A-1细胞相比，A549和95D细胞中NEDD9表达更高（P=0.003）。我们的结果表明，NEDD9在转移性肺腺癌和高侵袭性肺腺癌细胞系中上调，提示其可能参与调节细胞迁移和侵袭。
