The Emergency Department, People's Hospital of Zhengzhou, Zhengzhou, Henan, People's Republic of China.
Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.
Onco Targets Ther. 2015 Mar 10;8:633-40. doi: 10.2147/OTT.S77904. eCollection 2015.
To investigate the protein and mRNA expression of NEDD9 in gastric cancer (GC) tissues, adjacent atypical hyperplasia tissues, and normal gastric mucosa tissues, and analyze its relationship with the pathological features and prognosis of GC.
Forty cases of GC tissues, 20 cases of adjacent atypical hyperplasia tissues, and 40 cases of normal gastric mucous tissues were collected. Immunohistochemistry and Western blot were used to examine the expression of NEDD9 protein in various tissues. Situ hybridization and reverse transcription polymerase chain reaction were applied to detect the expression of NEDD9 mRNA in various tissues. The correlation of NEDD9 expression with invasion and metastasis of GC was analyzed.
The protein expression level of NEDD9 was significantly higher in GC tissues than in adjacent atypical hyperplasia tissues and normal gastric mucous tissues (P<0.05). The protein expression level of NEDD9 was positively related to the invasion depth of carcinoma and tumor lymph node metastasis (P<0.05), but unrelated to age, sex, tumor size, and clinical classification of cancer (P<0.05). The mRNA expression level of NEDD9 was also significantly higher in GC tissues than in adjacent atypical hyperplasia tissues and normal gastric mucous tissues (P<0.05), and positively related with the tumor lymph node metastasis and invasion depth of carcinoma (P<0.05).
NEDD9 is involved in the occurrence and development of GC, and it may be an important biological marker of GC metastasis and infiltration.
研究 NEDD9 在胃癌（GC）组织、癌旁非典型增生组织和正常胃黏膜组织中的蛋白和 mRNA 表达，并分析其与 GC 病理特征和预后的关系。
收集 40 例 GC 组织、20 例癌旁非典型增生组织和 40 例正常胃黏膜组织。采用免疫组化和 Western blot 检测 NEDD9 蛋白在各组织中的表达，采用原位杂交和反转录聚合酶链反应检测 NEDD9 mRNA 在各组织中的表达。分析 NEDD9 表达与 GC 侵袭和转移的相关性。
GC 组织中 NEDD9 蛋白表达水平明显高于癌旁非典型增生组织和正常胃黏膜组织（P<0.05）。NEDD9 蛋白表达水平与癌的浸润深度和肿瘤淋巴结转移呈正相关（P<0.05），但与年龄、性别、肿瘤大小和癌症临床分期无关（P<0.05）。GC 组织中 NEDD9 mRNA 表达水平也明显高于癌旁非典型增生组织和正常胃黏膜组织（P<0.05），且与肿瘤淋巴结转移和癌的浸润深度呈正相关（P<0.05）。
NEDD9 参与 GC 的发生发展，可能是 GC 转移和浸润的重要生物学标志物。
