An Peng, Sun Wan-Sen, Wu Xi-Li, Shi Xing-Min, Wang Zhu
Deoartment of Integrated Chinese and Western Medicine, Second Affiliated Hospital of Xi' an Jiaotong University, Xi'an 710004, China.
Zhongguo Zhen Jiu. 2012 Sep;32(9):819-23.
To observe the effect of acupuncture on cationized bovine serum albumin (C-BSA) nephritis model in rabbits and to explore its mechanism.
Fifty rabbits were randomly divided into a blank group, a model group, a metoprolol group, a irbesartan group and an acupuncture group, 10 rabbits in each group. The model was established by ear vein intravenous injection with C-BSA. The positive control groups were treated by intragastric administrated with metoprolol and irbesartan, respectively. The acupuncture group was treated by acupuncture at "Fengmen" (BL 12) and "Shenshu" (BL 23). No interventions were added on the blank group and the model group. The changes of blood pressure (BP), heart rate (HR), plasma norepinephrine (NE), serum creatinine (Scr), blood urea nitrogen (BUN) and 24 hours urine protein (24 h UP) in rabbits at the time points of 3rd, 6th and 8th week of treatment were observed.
After the model was established, the Scr of (194.30 +/- 20.09) micromol/L, BUN of (9.19 +/- 0.66) mmol/L and 24 h UP of (277.70 +/- 20.09) mg/24 h in the model group were all higher than the Scr of (66.03 +/- 4. 76) micromol/L, BUN of (4.11 +/- 0.71) mmol/L and 24 h UP of (14.28 +/- 1. 47) mg/24 h in the blank group (all P < 0.01), and the diffused mesenteria hyperplasia and the increase of intercapillary cells in the model group were showed in the pathological sections. After 3 weeks of treatment. The Scr of (99.82 +/- 9.29) micromol/L, BUN of (6.32 +/- 0.75) mmol/L and 24 h UP of (189.67 +/- 15.45) mg/ 24 h in the acupuncture group were all decreased significantly, furthermore, the decrease of BP, HR, NE were better than the other treatment groups (P < 0.05, P < 0.01). Except the level of 24 h up and HR at 8th week, other results were as same as the 3rd week.
Acupuncture can improve the function of kidney, decrease the content of 24 h UP and the underlying therapeutic mechanism could be correlated with that acupuncture can lower excitability of sympathetic nerve and alleviate the renal pathological lesion induced by nephritis.
观察针刺对兔阳离子化牛血清白蛋白（C-BSA）肾炎模型的影响并探讨其机制。
50只家兔随机分为空白组、模型组、美托洛尔组、厄贝沙坦组和针刺组，每组10只。通过耳静脉注射C-BSA建立模型。阳性对照组分别给予美托洛尔和厄贝沙坦灌胃治疗。针刺组针刺“风门”（BL 12）和“肾俞”（BL 23）。空白组和模型组不做干预。观察治疗第3、6、8周时家兔血压（BP）、心率（HR）、血浆去甲肾上腺素（NE）、血清肌酐（Scr）、血尿素氮（BUN）及24小时尿蛋白（24 h UP）的变化。
造模后，模型组Scr为（194.30±20.09）μmol/L、BUN为（9.19±0.66）mmol/L、24 h UP为（277.70±20.09）mg/24 h，均高于空白组的Scr（66.03±4.76）μmol/L、BUN（4.11±0.71）mmol/L、24 h UP（14.28±1.47）mg/24 h（均P<0.01），病理切片显示模型组系膜弥漫性增生，毛细血管间细胞增多。治疗3周后，针刺组Scr为（99.82±9.29）μmol/L、BUN为（6.32±0.75）mmol/L、24 h UP为（189.67±15.45）mg/24 h均显著降低，此外，血压、心率、NE的降低优于其他治疗组（P<0.05，P<0.01）。除第8周24 h up及心率水平外，其他结果与第3周相同。
针刺可改善肾功能，降低24 h UP含量，其潜在治疗机制可能与针刺能降低交感神经兴奋性、减轻肾炎所致肾脏病理损害有关。
