Department of Food and Nutrition, Hallym University, Chuncheon, Korea.
Am J Chin Med. 2012;40(6):1289-305. doi: 10.1142/S0192415X12500954.
Bone integrity is maintained through a balance between bone formation by osteoblasts and bone resorption by osteoclasts. Imbalance of the process results in metabolic bone diseases such as osteoporosis. This study investigated the yellow flag iris extract (YFIE) and revealed its anti-osteoporotic effects in osteoblastic MC3T3-E1 mouse cells and RAW 264.7 murine macrophages. When osteoblasts were treated with 1-20 μg/ml YFIE in an osteogenic medium, the bone nodule formation by calcium deposits was markedly enhanced during differentiation. Consistently, YFIE stimulated alkaline phosphatase activity and collagen type I secretion with a substantial effect on osteoblast proliferation. On the other hand, RAW 264.7 macrophages were pre-incubated with 1-20 μg/ml YFIE for 5 days in the presence of receptor activator of nuclear factor-κB ligand (RANKL). Non-toxic YFIE markedly attenuated the differentiation of macrophages to multi-nucleated osteoclasts. YFIE diminished RANKL-elevated tartrate-resistant acid phosphatase activity and bone resorption. In addition, the YFIE treatment retarded RANKL-induced cathepsin K production and carbonic anhydrase II expression, both of which are involved in bone resorption. Therefore, YFIE potentially posesses therapeutic agents that may prevent osteoporosis through promoting bone formation and reducing bone resorption.
骨完整性通过成骨细胞形成骨和破骨细胞吸收骨之间的平衡来维持。该过程的失衡导致代谢性骨病，如骨质疏松症。本研究调查了黄菖蒲提取物（YFIE），并揭示了其在成骨细胞 MC3T3-E1 小鼠细胞和 RAW 264.7 鼠巨噬细胞中的抗骨质疏松作用。当成骨细胞在成骨培养基中用 1-20μg/ml YFIE 处理时，钙沉积形成的骨结节形成在分化过程中明显增强。一致地，YFIE 刺激碱性磷酸酶活性和 I 型胶原分泌，并对成骨细胞增殖有显著影响。另一方面，RAW 264.7 巨噬细胞在用核因子-κB 配体（RANKL）预孵育 5 天的情况下用 1-20μg/ml YFIE 预处理。无毒的 YFIE 显著减弱了巨噬细胞向多核破骨细胞的分化。YFIE 降低了 RANKL 升高的抗酒石酸酸性磷酸酶活性和骨吸收。此外，YFIE 处理延迟了 RANKL 诱导的组织蛋白酶 K 产生和碳酸酐酶 II 表达，这两者都参与骨吸收。因此，YFIE 可能具有通过促进骨形成和减少骨吸收来预防骨质疏松症的治疗剂。
