载铁超顺磁性纳米粒子增强磁共振成像检测 AβPP/PS-1 转基因小鼠脑内阿尔茨海默病斑块。
SPION-enhanced magnetic resonance imaging of Alzheimer's disease plaques in AβPP/PS-1 transgenic mouse brain.
机构信息
Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
出版信息
J Alzheimers Dis. 2013;34(2):349-65. doi: 10.3233/JAD-121171.
Abstract
In our program to develop non-invasive magnetic resonance imaging (MRI) methods for the diagnosis of Alzheimer's disease (AD), we have synthesized antibody-conjugated, superparamagnetic iron oxide nanoparticles (SPIONs) for use as an in vivo agent for MRI detection of amyloid-β plaques in AD. Here we report studies in AβPP/PS1 transgenic mice, which demonstrate the ability of novel anti-AβPP conjugated SPIONs to penetrate the blood-brain barrier to act as a contrast agent for MR imaging of plaques. The conspicuity of the plaques increased from an average Z-score of 5.1 ± 0.5 to 8.3 ± 0.2 when the plaque contrast to noise ratio was compared in control AD mice with AD mice treated with SPIONs. The number of MRI-visible plaques per brain increased from 347 ± 45 in the control AD mice, to 668 ± 86 in the SPION treated mice. These results indicated that our SPION enhanced amyloid-β detection method delivers an efficacious, non-invasive MRI detection method in transgenic mice.
摘要
在我们开发用于阿尔茨海默病（AD）诊断的非侵入性磁共振成像（MRI）方法的计划中，我们合成了抗体偶联的超顺磁性氧化铁纳米颗粒（SPION），用作体内试剂，用于 MRI 检测 AD 中的淀粉样β斑块。在这里，我们报告了在 AβPP/PS1 转基因小鼠中的研究，这些研究表明新型抗 AβPP 偶联 SPION 能够穿透血脑屏障，作为斑块的磁共振成像对比剂发挥作用。当将斑块的对比噪声比与对照 AD 小鼠中的 AD 小鼠进行比较时，斑块的显著性从平均 Z 评分 5.1±0.5 增加到 8.3±0.2。在对照 AD 小鼠中每只脑的 MRI 可见斑块数量从 347±45 增加到 SPION 处理小鼠中的 668±86。这些结果表明，我们的 SPION 增强的淀粉样β检测方法在转基因小鼠中提供了一种有效、非侵入性的 MRI 检测方法。
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